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Packaging systems for human recombinant adenovirus to be used in gene therapy

  • US 6,306,652 B1
  • Filed: 06/15/1999
  • Issued: 10/23/2001
  • Est. Priority Date: 06/15/1995
  • Status: Expired due to Term
First Claim
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1. A method for producing a replication defective adenovirus comprising a gene of interest, without the concomitant production of replication competent adenovirus through homologous recombination, said method comprising:

  • a) providing a cell, said cell harboring a first adenoviral nucleic acid encoding adenoviral E1A and E1B gene products, and wherein said first adenoviral nucleic acid further comprises a deletion starting directley after a nucleotide after the furthest downstream E1B stop codon;

    b) transferring a recombinant nucleic acid into said cell, said recombinant nucleic acid comprising;

    a second adenoviral nucleic acid, and comprising at least one functional encapsidating signal, and at least two functional Inverted Terminal Repeats, said recombinant nucleic acid lacking overlapping sequences with the first adenoviral nucleic acid leading to replication competent adenovirus;

    c) culturing said cell; and

    d) harvesting replication defective adenovirus produced from said cell.

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