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Seeding parenchymal cells into compression resistant porous scaffold after vascularizing in vivo

  • US 6,309,635 B1
  • Filed: 11/28/1994
  • Issued: 10/30/2001
  • Est. Priority Date: 11/20/1986
  • Status: Expired due to Term
First Claim
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1. A method enhancing in vivo survival of parenchymal cells in an implanted scaffold comprising:

  • a. implanting into the body of a patient a porous three-dimensional scaffold composed of a biocompatible polymer and having generally interconnected pores of between approximately 100 and 300 microns in diameter throughout the scaffold, wherein the pores of the scaffold provide sufficient surface area to the scaffold to permit attachment of an amount of the cells effective to produce functional vascularized organ tissue in vivo, wherein the scaffold is resistant to compression within the patient, to thereby maintain the pore size of the scaffold between approximately 100 and 300 microns, and wherein the structure of the scaffold allows the introduction of cells into the vascularized scaffold without damage to the cells or patient;

    b. maintaining the scaffold in the patient until the scaffold is between 10% and 90% vascularized and infiltrated with viable connective tissue; and

    c. introducing viable parenchymal cells into the vascularized scaffold, wherein survival of the parenchymal cells in the vascularized scaffold is enhanced relative to survival of parenchymal cells in an unvascularized scaffold.

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