Fluorescent nanocrystal-labeled microspheres for fluorescence analyses

• US 6,309,701 B1
• Filed: 05/24/2000
• Issued: 10/30/2001
• Est. Priority Date: 11/10/1998
• Status: Expired due to Term

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Bio-Pixels, Ltd.
• Inventors: Barbera-Guillem, Emilio
• Primary Examiner(s): Nutter, Nathan M.
• Application Number:
  US09/577,761
• Time in Patent Office: 524 Days
• Field of Search: 427/213.3, 427/214, 427/215, 427/220, 428/402.24, 428/404, 424/9.1, 424/9.32, 424/9.36, 424/9.42, 424/9.6, 257/65, 257/614, 257/642
• US Class Current: 427/213.3
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07F 3/06 Zinc compounds
• CPC Class Codes: C07F 3/08 Cadmium compounds
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: Y10T 428/2989 Microcapsule with solid core [includes liposome]
• CPC Class Codes: Y10T 428/2993 Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]

Abstract

Provided are a fluorescent microsphere comprised of a plurality of fluorescent nanocrystals operably bound to a polymeric microsphere, and a method of producing the fluorescent microspheres which comprises contacting the polymeric microsphere with a plurality of fluorescent nanocrystals under suitable conditions in which the fluorescent naocrystals become operably bound to the polymeric microsphere. Also provided is a method of using the fluorescent microspheres capable of determining the presence or absence of a predetermined number of analytes in a sample by contacting the sample with the fluorescent microspheres, and detecting the fluorescence signal pattern of excited fluorescent microspheres bound to one or more analytes of the predetermined number of analytes, if present in the sample.

Claims

1. A fluorescent microsphere comprised of a plurality of fluorescent nanocrystals operably bound to a polymeric microsphere, wherein the fluorescent nanocrystals are functionalized with a plurality of molecules selected from the group consisting of carboxylic acid, diaminocarboxylic acid, monoaminocarboxylic acid, and a combination thereof.

2. The fluorescent microsphere according to claim 1, wherein the polymeric microsphere comprises multiple molecules of reactive functionality comprising a free chemical group, and the fluorescent nanocrystals comprise multiple molecules of reactive functionality comprising a reactive chemical group which are operably bound to the reactive functionality of the polymeric microsphere.

3. The fluorescent microsphere according to claim 1, wherein operably bound to the polymeric microsphere is a homogenous population of fluorescent nanocrystals.

4. The fluorescent microsphere according to claim 1, wherein operably bound to the polymeric microsphere is a heterogeneous population of fluorescent nanocrystals.

5. The fluorescent microsphere according to claim 1, further comprising affinity ligand operably bound thereto.

6. The fluorescent microsphere according to claim 1, wherein the fluorescent nanocrystals have an average particle size of approximately 1 nm to approximately 20 nm.

7. The fluorescent microsphere according to claim 1, wherein the fluorescent nanocrystals comprise semiconductor nanocrystals.

8. The fluorescent microsphere according to claim 7, wherein the fluorescent nanocrystals have a particle size that varies by less than 4%.

9. The fluorescent microsphere according to claim 1, wherein the fluorescent nanocrystals comprise doped metal oxide nanocrystals.

10. The fluorescent microsphere according to claim 9, wherein the fluorescent microsphere is magnetic.

11. The fluorescent microsphere according to claim 1, wherein the polymeric microsphere comprises a material selected from the group consisting of latex, acrylic, a glass/polymer composite, a thermoplastic, a polymeric material in combination with a magnetic material, and a combination thereof.

12. The fluorescent microsphere according to claim 11, wherein the polymeric microsphere further comprises carbon black in a weight percentage of from about 0.5 to about 5.

13. The fluorescent microsphere according to claim 1, wherein the polymeric microsphere comprises a combination of a polymeric material and a magnetic material selected from the group consisting of a polymeric material into which is embedded the magnetic material, a magnetic material core and a polymeric material coating, a polymeric material core with a magnetic material layer thereon and a polymeric material coating over the magnetic layer, and a magnetic material dispersed within a polymeric material.

14. The fluorescent microsphere according to claim 3, wherein the homogenous population of fluorescent nanocrystals, when excited with an excitation light source, emit a fluorescence signal pattern.

15. The fluorescent microsphere according to claim 4, wherein the heterogeneous population of fluorescent nanocrystals, when excited with an excitation light source, emit a fluorescence signal pattern.

16. The fluorescent microsphere according to claim 15, wherein the fluorescence signal pattern comprises multicolor fluorescence and detectably distinguishable intensities.

17. The fluorescent microsphere according to claim 16, wherein a code representative of the fluorescence signal pattern, and of the fluorescent microsphere which emits the fluorescence signal pattern, comprises an identifier for each color comprising the fluorescence signal pattern, and an identifier for the intensity of each color.

18. The fluorescent microsphere according to claim 17, wherein the code comprises a string of numbers.

19. A code representative of the fluorescence microsphere according to claim 17.

20. A code representative of the fluorescence microsphere according to claim 18.

21. A method of producing a fluorescent microsphere comprised of a plurality of fluorescent nanocrystals operably bound to a polymeric microsphere, wherein the method comprises contacting the polymeric microsphere with a plurality of fluorescent nanocrystals under suitable conditions for the plurality of fluorescent nanocrystals to become operably bound to the polymeric microsphere.

22. The method according to claim 21, wherein the polymeric microsphere comprises multiple molecules of reactive functionality comprising a free chemical group, and the fluorescent nanocrystals comprise multiple molecules of reactive functionality comprising a reactive chemical group which become operably bound to the reactive functionality of the polymeric microsphere.

23. The method according to claim 21, wherein operably bound to the polymeric microsphere is a homogenous population of fluorescent nanocrystals.

24. The method according to claim 21, wherein operably bound to the polymeric microsphere is a heterogeneous population of fluorescent nanocrystals.

25. The method according to claim 21, wherein the fluorescent microsphere further comprises affinity ligand operably bound thereto.

26. The method according to claim 21, wherein the fluorescent nanocrystals have an average particle size of approximately 1 nm to approximately 20 nm.

27. The method according to claim 21, wherein the fluorescent nanocrystals comprise semiconductor nanocrystals.

28. The method according to claim 21, wherein the fluorescent nanocrystals have a particle size that varies by less than 4%.

29. The method according to claim 21, wherein the fluorescent nanocrystals comprise doped metal oxide nanocrystals.

30. The method according to claim 29, wherein the fluorescent microsphere is magnetic.

31. The method according to claim 21, wherein the polymeric microsphere comprises a material selected from the group consisting of latex, acrylic, a glass/polymer composite, a thermoplastic, a polymeric material in combination with a magnetic material, and a combination thereof.

32. The method according to claim 21, wherein the polymeric microsphere further comprises carbon black in a weight percentage of from about 0.5 to about 5.

33. The method according to claim 21, wherein the polymeric microsphere comprises a combination of a polymeric material and a magnetic material selected from the group consisting of a polymeric material into which is embedded the magnetic material, a magnetic material core and a polymeric material coating, a polymeric material core with a magnetic material layer thereon and a polymeric material coating over the magnetic layer, and a magnetic material dispersed within a polymeric material.

34. A method of using fluorescent microspheres for determining the presence or absence of a predetermined number of analytes in a sample, wherein a fluorescent microsphere comprises a plurality of fluorescent nanocrystals operably bound to a polymeric microsphere and further comprises affinity ligand operably bound thereto, the method comprising:

(a) contacting the fluorescent microspheres with the sample under suitable conditions for the fluorescent microspheres to bind the predetermined number of analytes, if present in the sample, in forming complexes;

(b) exposing the complexes, if formed, to an excitation wavelength light source suitable for exciting the fluorescent microspheres of the complexes to emit a fluorescence signal pattern; and

(c) detecting the fluorescence signal pattern emitted by the complexes, if present, by a detection means for detecting the fluorescence signal pattern;

wherein detection of the fluorescent signal pattern is indicative of the presence of an analyte of the predetermined number of analytes.

35. The method according to claim 34, wherein the presence of an analyte is detected, and the method further comprises quantitating an amount of the analyte detected by measuring the intensity of the fluorescence signal pattern emitted from the fluorescent microspheres bound to the analyte.

36. The method according to claim 34, wherein the method is performed using a fluorescent detection system selected from the group consisting of an affinity-based assay, fluorescence-based immunoassay, fluorescent staining, flow cytometry, and a hybridization-based assay.

37. The method according to claim 34, wherein the fluorescence signal pattern comprises one or more fluorescence emission peaks, wherein each peak has a narrow spectral band in the range of from about 400 nm to about 800 nm.

38. The method according to claim 34, wherein the excitation wavelength light source for exciting the fluorescent microspheres is a wavelength spectrum in the range of about 300 nm to about 400 nm.

39. The method according to claim 34, wherein the detection means is selected from the group consisting of a photodetector, a filter, a charge couple device camera, a fluorimeter, a fluorescence microscope, a fluorescence cube, a computer for manipulating fluorescence signal pattern data collected, and a combination thereof.