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Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes

  • US 6,309,824 B1
  • Filed: 01/16/1997
  • Issued: 10/30/2001
  • Est. Priority Date: 01/16/1997
  • Status: Expired due to Fees
First Claim
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1. A method for analyzing a target nucleic acid, comprising the steps of:

  • providing an array comprising a substrate and a plurality of heterogenous mixtures of different oligonucleotide probes, wherein each of said mixtures is immobilized on the substrate at a distinct position with the array;

    providing a plurality of labeled oligonucleotide probes;

    contacting the target nucleic acid with the immobilized probes and the labeled probes under conditions that allow the immobilized probes and the labeled probes which form perfectly hybridized matches with the target nucleic acid to be distinguished from the immobilized probes and the labeled probes that hybridize with a one base mismatch to the target nucleic acid;

    covalently joining the immobilized probes and labeled probes that hybridize adjacent to each other with a target nucleic acid molecule identifying a plurality of covalently joined immobilized probes and labeled probes; and

    analyzing the nucleic acid from the identified probes.

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