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Compounds for protecting hydroxyls and methods for their use

  • US 6,309,836 B1
  • Filed: 10/05/1999
  • Issued: 10/30/2001
  • Est. Priority Date: 10/05/1999
  • Status: Expired due to Fees
First Claim
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1. A method for sequencing a nucleic acid comprising the steps of:

  • a) contacting a target nucleic acid with a primer under conditions wherein said primer anneals to said target nucleic acid in a sequence specific manner and wherein at least a portion of said primer is complementary to a portion of said target nucleic acid;

    b) incorporating a hydrocarbyldithiomethyl-modified nucleotide into said primer; and

    c) detecting incorporation of said hydrocarbyldithiomethyl-modified nucleotide, wherein said hydrocarbyldithiomethyl-modified nucleotide is complementary to said target nucleic acid at said hydrocarbyldithiomethyl-modified nucleotide'"'"'s site of incorporation thereby identifying the sequence of one nucleobase of said target nucleic acid.

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