Nucleotides and analogs having photoremoveable protecting groups
First Claim
Patent Images
1. A compound having a photoremoveable protecting group covalently attached to a C-3′
- or C-5′
oxygen atom, wherein said compound is selected from the group consisting of natural or unnatural nucleoside phosphoramidites and natural or unnatural nucleoside H-phosphonates.
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Abstract
A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.
265 Citations
17 Claims
-
1. A compound having a photoremoveable protecting group covalently attached to a C-3′
- or C-5′
oxygen atom, wherein said compound is selected from the group consisting of natural or unnatural nucleoside phosphoramidites and natural or unnatural nucleoside H-phosphonates. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
- or C-5′
-
17. A method of activating a terminal nucleotide in a nucleic acid, said terminal nucleotide having a photoremoveable protecting group, said method comprising removing said photoremoveable protecting group from a latent C5′
- hydroxy moiety in said nucleic acid.
Specification