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pH dependent ion exchange matrix and method of use in the isolation of nucleic acids

  • US 6,310,199 B1
  • Filed: 05/14/1999
  • Issued: 10/30/2001
  • Est. Priority Date: 05/14/1999
  • Status: Expired due to Term
First Claim
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1. A pH dependent ion exchange matrix for isolating a target nucleic acid, comprising:

  • a silica magnetic particle; and

    a plurality of first ion exchange ligands, each first ion exchange ligand comprising;

    an aromatic hydrocarbon ring, wherein at least one member of the ring is an amine with a pK of less than about 9;

    a spacer covalently attached to the aromatic hydrocarbon ring, the spacer comprising a spacer alkyl chain of with an amine terminus, and an acidic moiety covalently attached to the spacer alkyl chain; and

    a linker comprising a linker alkyl chain covalently attached to the silica magnetic particle through a silica residue at a first end of the linker alkyl chain and covalently attached to the amine terminus of the spacer at a second end of the linker alkyl chain;

    wherein the matrix has a capacity to adsorb to a target nucleic acid at a first pH, and to release the target nucleic acid at a desorption pH which is higher than the first pH.

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