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Methods and compositions for determining the sequence of nucleic acid molecules

  • US 6,312,893 B1
  • Filed: 07/22/1997
  • Issued: 11/06/2001
  • Est. Priority Date: 01/23/1996
  • Status: Expired due to Term
First Claim
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1. A method for determining the sequence of a nucleic acid molecule, comprising:

  • (a) generating tagged nucleic acid fragments which are complementary to a selected target nucleic acid molecule, wherein a tag is an organic moiety that is correlative with a particular nucleotide and detectable by non-fluorescent spectrometry or potentiometry;

    (b) separating the tagged fragments by sequential length;

    (c) cleaving the tags from the tagged fragments; and

    (d) detecting the tags by non-fluorescent spectrometry or potentiometry, and therefrom determining the sequence of the nucleic acid molecule.

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