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Assays for measuring nucleic acid binding proteins and enzyme activities

  • US 6,312,896 B1
  • Filed: 09/17/1998
  • Issued: 11/06/2001
  • Est. Priority Date: 09/17/1998
  • Status: Expired due to Fees
First Claim
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1. A method for assaying a sample for the presence of a nucleic acid binding protein, which comprises:

  • a) mixing at least one predetermined single- or double-stranded nucleic acid containing at least one label and containing a protein binding nucleotide sequence with a sample which may contain a nucleic acid binding protein, b) incubating the mixture of step a) under conditions which allow the binding of said nucleic acid binding protein to said at least one predetermined single- or double-stranded nucleic acid to form a complex, c) adding a nucleic acid-cleaving enzyme or reagent to the mixture of step b), d) incubating the mixture of step c) under conditions which allow the cleavage of said at least one predetermined single- or double-stranded nucleic acid which has not formed a complex, and e) measuring the amount of said complex by means that do not include gel electrophoretic separation to measure said nucleic acid binding protein.

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