Isolated DNA sequence which can serve as terminator region in a chimeric gene capable of being used for the transformation of plants
DC CAFCFirst Claim
1. An expression cassette for expression of a chimeric gene in plants, comprising in the direction of transcription, a DNA sequence for at least one terminator region of an Arabidopsis thaliana histone H4748 gene in direct or reverse orientation relative to the H4748 gene from which it is derived, wherein said terminator region regulates the expression of the chimeric gene.
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Abstract
Isolated DNA sequence which can serve as terminator region in a chimeric gene capable of being used for the transformation of plants
1) Chimeric gene for the transformation of plants.
2) It comprises at least, in the direction of transcription, a promoter region, a transgene and a terminator region, characterized in that the terminator region consists of at least one terminator region from a plant histone gene permitting the expression of the protein in the regions of rapid growth.
3) Production of transgenic plants.
331 Citations
18 Claims
- 1. An expression cassette for expression of a chimeric gene in plants, comprising in the direction of transcription, a DNA sequence for at least one terminator region of an Arabidopsis thaliana histone H4748 gene in direct or reverse orientation relative to the H4748 gene from which it is derived, wherein said terminator region regulates the expression of the chimeric gene.
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18. A 3′
- termination region isolated from an Arabidopsis thaliana histone H4 gene and having the nucleotide sequence as set forth in Sequence ID NO;
1.
- termination region isolated from an Arabidopsis thaliana histone H4 gene and having the nucleotide sequence as set forth in Sequence ID NO;
Specification