Flow cell for synthesis of arrays of DNA probes and the like
First Claim
1. A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:
- (a) a base having a central window opening and a registration surface against which a substrate with an active surface may be mounted;
(b) a gasket having a central opening defining an active area surrounded by material of the gasket, an inlet extension opening extending from the central opening, and an outlet extension opening extending from the central opening of the gasket, the gasket mountable on a substrate which is mounted on the base;
(c) a press block having an engagement surface which may be engaged against the gasket to press the gasket against a substrate mounted on the base; and
(d) means for pressing together the press block, gasket, and substrate to the base to form a sealed volume defined by the periphery of the openings in the gasket and the active surface of the substrate and the engagement surface of the press block, and an inlet channel in the press block extending from an exterior surface thereof to communication with the inlet extension opening in the gasket and an outlet channel in the press block extending from communication with the outlet extension opening in the gasket to an external surface of the press block.
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Accused Products
Abstract
A flow cell which can be used in the synthesis of DNA probes on an active surface of the substrate includes a base having a central window opening and a registration surface against which the substrate may be mounted. A gasket having a central opening defining an active area is mounted on the active surface of the substrate and has inlet and output extension openings which extend away from the central opening. A press block is engaged against the gasket to fully enclose an active volume between the press block, the peripheral walls of the central opening in the gasket, and the active surface of the substrate. A press is mounted to the base to selectively press the press block against the gasket and hold it in position. Channels in the press block extend to the extension openings in the gasket to allow flow of reagent into and out of the active volume, which is confined to the thickness of the gasket and can thus be minimized. The press may include a press screw which can be turned to engage against the press block to hold it into position, and which can be turned to release the press block from the gasket, allowing rapid and easy replacement of substrates.
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Citations
21 Claims
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1. A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:
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(a) a base having a central window opening and a registration surface against which a substrate with an active surface may be mounted;
(b) a gasket having a central opening defining an active area surrounded by material of the gasket, an inlet extension opening extending from the central opening, and an outlet extension opening extending from the central opening of the gasket, the gasket mountable on a substrate which is mounted on the base;
(c) a press block having an engagement surface which may be engaged against the gasket to press the gasket against a substrate mounted on the base; and
(d) means for pressing together the press block, gasket, and substrate to the base to form a sealed volume defined by the periphery of the openings in the gasket and the active surface of the substrate and the engagement surface of the press block, and an inlet channel in the press block extending from an exterior surface thereof to communication with the inlet extension opening in the gasket and an outlet channel in the press block extending from communication with the outlet extension opening in the gasket to an external surface of the press block. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:
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(a) a base having a central window opening and a registration surface against which a substrate with an active surface may be mounted;
(b) a gasket having a central opening defining an active area surrounded by material of the gasket, an inlet extension opening extending from the central opening, and an outlet extension opening extending from the central opening of the gasket, the gasket mountable on a substrate which is mounted on the base;
(c) a press block having an engagement surface which may be engaged against the gasket to press the gasket against a substrate mounted on the base; and
(d) means for pressing together the press block, gasket, and substrate to the base to form a sealed volume defined by the periphery of the openings in the gasket and the active surface of the substrate and the engagement surface of the press block including a standing frame secured to the base having an upright section extending from the base and an arm section extending from the upright section over the window opening in the base, and a press screw threadingly engaged with the arm section with a drive end thereof positioned to engage against an external surface of the press block as the press screw is turned to thread it toward the press block, and an inlet channel in the press block extending from an exterior surface thereof to communication with the inlet extension opening in the gasket and an outlet channel in the press block extending from communication with the outlet extension opening in the gasket to an external surface of the press block. - View Dependent Claims (9, 10)
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11. A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:
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(a) a base having a central window opening and a registration surface against which a substrate with an active surface may be mounted;
(b) a press block having an engagement surface such that a substrate with an active surface can be engaged between the press block and the registration surface of the base;
(c) a press comprising a standing frame secured to the base and having an upright section extending from the base and an arm section extending from the upright section over the central window opening in the base, and a press screw threadingly engaged with the arm section and positioned to engage an external surface of the press block with a drive end thereof as the press screw is turned to thread it toward the press block. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification