Method of diagnosing of exposure to toxic agents by measuring distinct pattern in the levels of expression of specific genes
First Claim
1. A method of pre-symptom diagnosis of exposure to a toxic agent comprising:
- obtaining a sample of mammalian body fluid or tissue from a subject;
detecting a pattern of gene expression or protein expression present in said sample;
comparing the pattern of gene or protein expression from said sample with a library of known patterns of gene or protein expressions for toxic agents; and
determining whether the subject has been exposed to a toxic agent in said library and if said subject has been exposed to a toxic agent in said library, identifying said toxic agent based on information in said library.
2 Assignments
0 Petitions
Accused Products
Abstract
A method of diagnosing exposure to a toxic agent comprising the steps of detecting the amount of protein/gene expression present in a sample of mammalian tissue or mammalian body fluids that has not been exposed to a toxic agent. Then the amount of protein/gene expression present in a sample of mammalian tissue or mammalian body fluids that has been exposed to the toxic agent is detected. A determination of the difference in the detected amount of protein/gene expression between the exposed and unexposed samples is made. A comparison of the difference to a library of expected protein/gene expression for predetermined toxic agents is made. Finally, an evaluation is made whether the difference indicates the exposure to a particular toxic agent. A treatment method for administering a therapeutic agent which inhibits the mechanistic pathways necessary to maintain the progression of lethal shock is also disclosed.
-
Citations
20 Claims
-
1. A method of pre-symptom diagnosis of exposure to a toxic agent comprising:
-
obtaining a sample of mammalian body fluid or tissue from a subject;
detecting a pattern of gene expression or protein expression present in said sample;
comparing the pattern of gene or protein expression from said sample with a library of known patterns of gene or protein expressions for toxic agents; and
determining whether the subject has been exposed to a toxic agent in said library and if said subject has been exposed to a toxic agent in said library, identifying said toxic agent based on information in said library. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 17)
-
-
12. A method of diagnosing exposure to a toxic agent comprising the steps of:
-
detecting an amount of protein or gene expression present in a sample of mammalian tissue or mammalian body fluids that has been unexposed to the toxic agent;
detecting an amount of protein or gene expression present in a sample of mammalian tissue or mammalian body fluids that has been exposed to the toxic agent;
determining the difference in the detected amount of protein/gene expression between the exposed and unexposed samples;
comparing the difference to a library of expected protein/gene expression for predetermined toxic agents;
evaluating whether the difference indicates that said sample has been exposed to a particular toxic agent. - View Dependent Claims (13, 14, 15, 16)
-
-
18. A method of pre-symptom diagnosis of exposure to a toxic agent, wherein said toxic agent is SEB (staphyloccocal enterotoxin), Anthrax, Cholera, LPS (lipopolysaccharide), or Plague comprising:
-
obtaining a sample of mammalian body fluid or tissue from a subject;
detecting a pattern of gene expression or protein expression present in said sample;
comparing the pattern of gene or protein expression from said sample with a library of known patterns of gene or protein expressions for toxic agents; and
determining whether the subject has been exposed to said toxic agent and if said subject has been exposed to a toxic agent, identifying said toxic agent based on information in said library. - View Dependent Claims (19, 20)
-
Specification