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Polymerase extension at 3′ terminus of PNA-DNA chimera

  • US 6,316,230 B1
  • Filed: 08/13/1999
  • Issued: 11/13/2001
  • Est. Priority Date: 08/13/1999
  • Status: Expired due to Term
First Claim
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1. A method of producing a non-radioisotopically labelled chimeric extension product comprising the step of enzymatically extending a PNA-DNA chimera in the presence of a template nucleic acid, a polymerase and a primer extension reagent, wherein said primer extension reagent comprises a nucleotide 5′

  • -triphosphate capable of effecting enzymatic chimera primer extension, the PNA-DNA chimera or the nucleotide 5′

    -triphosphate is labelled with a non-radioisotopic label selected from the group consisting of a fluorescent dye, a fluorescence quencher, a hybridization stabilizer, an energy-transfer dye pair, an electrophoretic mobility modifier, a chemiluminescent dye, an amino acid, a protein, a peptide, an enzyme, and an affinity ligand, andthe PNA-DNA chimera has the structure;

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