Preparation and use of magnetically susceptible polymer particles

• US 6,316,235 B1
• Filed: 01/10/2000
• Issued: 11/13/2001
• Est. Priority Date: 05/26/1995
• Status: Expired due to Term

• Current Assignee: Klaus Mosbach
• Original Assignee: IGEN, Inc. (Teligent Incorporated)
• Inventors: Kriz, Dario, Ansell, Richard J., Mosbach, Klaus
• Primary Examiner(s): Naff, David M.
• Application Number:
  US09/480,637
• Time in Patent Office: 673 Days
• Field of Search: 435/174, 435/176, 435/177, 435/180, 435/182, 435/280, 210/222, 209/8, 436/525, 436/531, 252/63.51 R, 526/95
• US Class Current: 435/180
• CPC Class Codes: C07B 57/00 Separation of optically-active compounds
• CPC Class Codes: C08F 220/28 containing no aromatic rings in the alcohol moiety
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template

Abstract

Polymer particles are formed containing molecular memory recognition sites and magnetically susceptible components. The particles are prepared by co-polymerizing one or more monomers and a cross-linking agent in the presence of at least one imprint molecule and at least one magnetically susceptible component such as iron oxide or nickel oxide, and removing the imprint molecule to form molecular memory recognition sites. The particles are also prepared by co-polymerizing the monomer and cross-linking agent in the presence of the imprint molecule to produce particles, removing the imprint molecule and associating magnetically susceptible components with the particles. The particles may also be prepared containing selective adsorbents such as cells or antibodies. The particles are used for selective adsorption of a product such as separating and resolving two different enantiomeric forms due to one of the forms adsorbing to a memory recognition site created by using the form as an imprint molecule. A magnetic field is used to separate particles containing an adsorbed product from a solution in which the product is adsorbed to the particles.

Claims

1. Magnetically susceptible polymer particles, comprising

(a) molecular memory recognition sites, and

(b) magnetically susceptible components.

2. The particles of claim 1, wherein the magnetically susceptible components comprise one or more metal oxides.

3. The particles of claim 2, wherein the metal oxides are iron oxides or nickel oxides.

4. The particles of claim 2, wherein the metal oxides are iron oxides.

5. The particles of claim 2, wherein the metal oxides are nickel oxides.

6. Magnetically susceptible polymer particles, comprising the combination of

(a) molecular memory recognition sites, and

(b) magnetically susceptible components;

said magnetically susceptible particles being prepared by the process comprising;

(i) co-polymerizing one or more monomers and a cross-linking agent in the presence of at least one imprint molecule and at least one magnetically susceptible component; and

(ii) removing the imprint molecule to form the molecular memory recognition sites.

7. The particles of claim 6, wherein the monomers are at least one of methacrylic acid, methyl methacrylate, benzyl methacrylate, or styrene.

8. The particles of claim 6, wherein the cross-linking agent is ethylene glycol dimethacrylate or 1,1,1-tris(hydroxymethyl)propane triacrylate.

9. Magnetically susceptible polymer particles, comprising the combination of molecular memory recognition sites and magnetically susceptible components, said magnetically susceptible polymer particles prepared by the process comprising:

(a) co-polymerizing one or more monomers and a cross-linker agent in the presence of at least one imprint molecule to form a particle;

(b) removing the imprint molecule from the particle, and

(c) associating magnetically susceptible components with the particle.

10. The particles of claim 9 wherein the monomers are at least one of methacrylic acid, methyl methacrylate, benzyl methacrylate, or styrene.

11. The particles of claim 9, wherein the cross-linking agent is ethylene glycol dimethacrylate or 1,1,1-tris(hydroxymethyl)propane triacrylate.

12. A process for preparing magnetically susceptible polymer particles having molecular memory recognition sites, comprising polymerizing one or more monomers and a cross-linking agent in the presence of an imprint molecule and magnetically susceptible components and removing said imprint molecule to leave the molecular memory recognition site.