Polypeptides for promoting cell attachment
First Claim
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1. A prosthetic device provided with a coating composition comprising a polypeptide having a length of no more than about 100 amino acid residues that includes an amino acid residue sequence represented by the formula:
- AspArgXaa1ProHisXaa2Arg (SEQ ID NO
1), wherein Xaa1 and Xaa2 are any amino acid residue and said polypeptide binds GPIIb-IIIa in an ArgGlyAsp-independent manner.
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Abstract
Novel polypeptides derived from human fibronectin are described which bind to integrin receptors expressed by cells. The receptor binding site of human fibronectin begins at amino acid residue 1394 and ends at residue 1400 of fibronectin. The polypeptides facilitate attachment of cells to substrates either alone or in conjunction with RGD-containing peptides. Vectors, fusion proteins and antibodies are also described. Methods for promoting cell attachment and for inhibiting cell adhesion are also described.
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Citations
12 Claims
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1. A prosthetic device provided with a coating composition comprising a polypeptide having a length of no more than about 100 amino acid residues that includes an amino acid residue sequence represented by the formula:
- AspArgXaa1ProHisXaa2Arg (SEQ ID NO
1), wherein Xaa1 and Xaa2 are any amino acid residue and said polypeptide binds GPIIb-IIIa in an ArgGlyAsp-independent manner. - View Dependent Claims (2)
- AspArgXaa1ProHisXaa2Arg (SEQ ID NO
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3. A prosthetic device provided with a coating composition comprising a polypeptide having a length of no more than about 100 amino acid residues that includes an amino acid residue sequence—
- AspArgXaa1ProHisXaa2ArgXaa3Xaa4Xaa5Xaa6—
(SEQ ID NO
2), wherein Xaa1 is Val or Ala, Xaa2 is Ser or Ala, Xaa3 is Asn or Ala, Xaa4 is Ser or Ala, Xaa5 is Ile or Ala, and Xaa6 is Thr or Ala. - View Dependent Claims (4)
- AspArgXaa1ProHisXaa2ArgXaa3Xaa4Xaa5Xaa6—
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5. A skin graft provided with a coating composition comprising a polypeptide having a length of no more than about 100 amino acid residues that includes an amino acid residue sequence represented by the formula:
- AspArgXaa1ProHisXaa2Arg (SEQ ID NO
1), wherein Xaa1 and Xaa2 are any amino acid residue and said polypeptide binds GPIIb-IIIa in an ArgGlyAsp-independent manner. - View Dependent Claims (6)
- AspArgXaa1ProHisXaa2Arg (SEQ ID NO
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7. A skin graft provided with a coating composition comprising a polypeptide having a length of no more than about 100 amino acid residues that includes an amino acid residue sequence—
- AspArgXaa1ProHisXaa2ArgXaa3Xaa4Xaa5Xaa6—
(SEQ ID NO
2), wherein Xaa1 is Val or Ala, Xaa2 is Ser or Ala, Xaa3 is Asn or Ala, Xaa4 is Ser or Ala, Xaa5 is Ile or Ala, and Xaa6 is Thr or Ala. - View Dependent Claims (8)
- AspArgXaa1ProHisXaa2ArgXaa3Xaa4Xaa5Xaa6—
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9. An antibody composition that immunoreacts with fibronectin and with a polypeptide having a length of no more than about 100 amino acid residues that includes an amino acid residue sequence represented by the formula:
- AspArgXaa1ProHisXaa2Arg (SEQ ID NO
1), wherein Xaa1 and Xaa2 are any amino acid residue and said polypeptide binds GPIIb-IIIa in an ArgGlyAsp-independent manner. - View Dependent Claims (10)
- AspArgXaa1ProHisXaa2Arg (SEQ ID NO
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11. An antibody composition that immunoreacts with fibronectin and with a polypeptide having a length of no more than about 100 amino acid residues that includes an amino acid residue sequence—
- AspArgXaa1ProHisXaa2ArgXaa3Xaa4Xaa5Xaa6—
(SEQ ID NO
2), wherein Xaa1 is Val or Ala, Xaa2 is Ser or Ala, Xaa3 is Asn or Ala, Xaa4 is Ser or Ala, Xaa5 is Ile or Ala, and Xaa6 is Thr or Ala. - View Dependent Claims (12)
- AspArgXaa1ProHisXaa2ArgXaa3Xaa4Xaa5Xaa6—
Specification