Fluid sample testing system

• US 6,318,191 B1
• Filed: 06/23/1999
• Issued: 11/20/2001
• Est. Priority Date: 06/24/1998
• Status: Expired due to Term

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: CHEN Ching-Too
• Inventors: Chen, Shuqi
• Primary Examiner(s): Kwok, Helen
• Assistant Examiner(s): Loo, Dennis
• Application Number:
  US09/339,056
• Time in Patent Office: 881 Days
• Field of Search: 738/631.1, 738/642.1, 422/68.1, 422/82, 422/82.05, 422/82.06, 277/314, 206/438, 206/534.1, 206/539, 425/5, 534/63
• US Class Current: 73/863.11
• CPC Class Codes: B01F 31/55 the materials to be mixed being contained in a flexible bag submitted to periodical deformation
• CPC Class Codes: B01F 33/452 using independent floating stirring elements
• CPC Class Codes: B01F 35/75425 using pistons or plungers
• CPC Class Codes: B01F 35/754251 reciprocating in the mixing receptacle
• CPC Class Codes: B01F 35/7543 using pneumatic pressure, overpressure or gas pressure in a closed receptacle or circuit system
• CPC Class Codes: B01F 35/7546 using squeezing means on a deformable container
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/50 Containers for the purpose of retaining a material to be analysed, e.g. test tubes devices for taking samples of blood A61B5/15
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: G01N 35/00009 provided with a sample supporting tape, e.g. with absorbent zones
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream

Abstract

A sample testing system has a chamber sealing, apparatus adapted to receive a chamber containing a fluid sample material and to seal portions of a chamber into segments. A reagent injector cartridge actuator is adapted to receive a reagent injector cartridge having at least one needle in fluid communication with a reagent reservoir, and to move a reagent injector cartridge to inject a quantity of reagent into a segment of a chamber. In certain preferred embodiments, a flow control device induces a flow of sample in a segment. A sensor generates a signal corresponding to a condition of the mixture.

Claims

1. A sample testing system comprising, in combination:

a chamber sealing apparatus to form a plurality of seals defining a plurality of fluid-tight segments of the chamber;

a reagent injector cartridge actuator adapted to receive a reagent injector cartridge having at least one needle in fluid communication with a reagent reservoir, and to move a reagent injector cartridge to inject a quantity of reagent into a segment of a chamber;

a flow control device comprising at least one contact member movable into contact with a segment of a chamber to effect mechanically induced fluid flow within a flow passageway in a segment of a chamber; and

a sensor to generate an output signal corresponding to a condition of a fluid sample material within a segment of a chamber.

2. The sample testing system of claim 1, further comprising an output device responsive to the output signal.

3. The sample testing system of claim 1, wherein the sensor comprises a light detector to receive light responsive to a fluid sample in a segment of a chamber and to generate an output signal corresponding to a condition of a fluid sample material within a segment of a chamber.

4. The sample testing system of claim 1, further comprising a sensor to receive information from a label of a reagent injector cartridge.

5. The sample testing system according to claim 1, further comprising a programmable control system coupled with the chamber sealing apparatus, the reagent injector cartridge actuator, the flow control device, and the sensor.

6. The sample testing system according to claim 1, wherein the chamber is a flexible plastic tube containing a fluid sample material and the chamber sealing apparatus has a tube compression and sealing member to laterally seal the flexible plastic tube and thereby form a fluid-tight tubule containing a portion of the fluid sample material between axial spaced lateral seals.

7. The sample testing system according to claim 6, wherein the chamber sealing apparatus comprises:

a first sealing head comprising the tube compression and sealing member; and

a second sealing head, at least one of the first and second sealing heads being movable toward the other of the sealing heads to compress a section of flexible plastic tube positioned between the first and second sealing heads to create a sample-free zone in the tube, wherein the tube compression and sealing member is operatively connected to a power source to heat a sealing zone of tube located in the sample-free zone to form a fluid-tight lateral seal in the tube.

8. The sample testing system according to claim 7, wherein the first sealing head and the second head each has at least one electrically conductive portion and at least one of the sealing heads has a substantially convex profile.

9. The sample testing system according to claim 7, further comprising a radio frequency power generator to transmit radio frequency energy between the first and second sealing heads through the sealing zone to form a lateral seal in the tube.

10. The sample testing system according to claim 7, wherein the chamber sealing apparatus further comprises a compression apparatus to partially compress a portion of a tube to create a tubule of a predetermined volume.

11. The sample testing system according to claim 1, wherein the flow control device further comprises a base member for supporting a segment of a chamber, the contact member being movable toward the base member to partially compress at least one portion of a segment of a chamber against the base member forming a fluid passageway in the segment and further movable in repeating fashion to alternately compress and decompress at least one portion of the segment to induce fluid flow within the fluid passageway.

12. The sample testing system according to claim 11, wherein the sensor detects light passing through a segment of a chamber as the flow control device is operating to induce fluid flow in that segment.

13. The sample testing system according to claim 1, further comprising an incubation chamber to retain a segment of a chamber for a predetermined period of time.

14. The sample testing system according to claim 13, wherein the incubation chamber includes a temperature control device to control the temperature of a segment of a chamber in the incubation chamber.

15. The sample testing system according to claim 1, wherein the sensor detects properties of particles within a fluid sample in a segment of a chamber.

16. The sample testing system according to claim 1, further comprising a light source to project light through a segment of a chamber.

17. The sample testing system according to claim 16, wherein the sensor comprises a camera having a microscopic lens to capture images of a fluid sample in an inspection zone of a segment of chamber as the light source is projecting light through the inspection zone;

and

further comprising a computer operably connected to the camera to analyze the images of the fluid sample.

18. The sample testing system according to claim 1, further comprising

a pair of electrodes adapted to have a predetermined voltage difference, and

an electrode actuator to insert the pair of electrodes into a segment of a chamber, wherein the sensor is responsive to electrophoretic light emitted from within a segment of a chamber.

19. The sample testing system according to claim 18, wherein one of the electrodes comprises a fiber optic conductor.

20. The sample testing system according to claim 1, further comprising a segment of a chamber and a coating on an outside surface of the segment to increase the transmission of light through the segment.

21. The sample testing system according to claim 20, wherein the coating has a refractive index substantially the same as a refractive index of the segment.

22. The sample testing system according to claim 1, further comprising a segment advancing device to transport a length of tube within the sample testing system.

23. The sample testing system according to claim 22, wherein the segment advancing device comprises a pair of rotatable wheels spaced apart from one another to receive the tube therebetween, at least one of the wheels being a driven wheel operatively connected to an output member of an electric motor.

24. A sample testing system comprising, in combination:

tube sealing apparatus having a tube compression and sealing member to laterally seal a flexible plastic tube containing a fluid sample material, whereby a fluid-tight tubule containing a portion of the fluid sample material can be formed between axially spaced lateral seals;

the tube sealing apparatus comprising a first sealing head comprising the tube compression and sealing member; and

a second sealing head, at least one of the first and second sealing heads being movable toward the other of the sealing heads to compress a section of flexible plastic tube positioned between the first and second sealing heads to create a sample-free zone in the tube, wherein the tube compression and sealing member is operatively connected to a power source to heat a sealing zone of tube located in the sample-free zone to form a fluid-tight lateral seal in the tube;

a reagent injector cartridge actuator adapted to receive a reagent injector cartridge having at least one needle in fluid communication with a reagent reservoir, and to move a reagent injector cartridge to inject a quantity of reagent into a fluid-tight tubule; and

a sensor to generate an output signal corresponding to a condition of a fluid sample material within a fluid-tight tubule.

25. The sample testing system according to claim 24, wherein the first sealing head and the second head each has at least one electrically conductive portion and at least one of the sealing heads has a substantially convex profile.

26. The sample testing system according to claim 24, further comprising a radio frequency power generator to transmit radio frequency energy between the first and second sealing heads through the sealing zone to form a lateral seal in the tube.

27. The sample testing system according to claim 24, wherein the chamber sealing apparatus further comprises a compression apparatus to partially compress a portion of a tube to create a tubule of a predetermined volume.

28. A sample testing system comprising, in combination:

a chamber sealing apparatus to form a plurality of seals defining a plurality of fluid-tight segments of the chamber;

a reagent injector cartridge actuator adapted to receive a reagent injector cartridge having at least one needle in fluid communication with a reagent reservoir, and to move a reagent injector cartridge to inject a quantity of reagent into a segment of a chamber;

a sensor to generate an output signal corresponding to a condition of a fluid sample material within a segment of a chamber;

a pair of electrodes adapted to have a predetermined voltage difference, and

an electrode actuator to insert the pair of electrodes into a segment of a chamber, wherein the sensor is responsive to electrophoretic light emitted from within a segment of a chamber.

29. The sample testing system according to claim 28, wherein one of the electrodes comprises a fiber optic conductor.

30. A sample testing system comprising, in combination:

a chamber sealing apparatus to form a plurality of seals defining a plurality of fluid-tight segments of the chamber;

a segment of the chamber including a coating on an outside surface of the segment to increase the transmission of light through the segment;

a reagent injector cartridge actuator adapted to receive a reagent injector cartridge having at least one needle in fluid communication with a reagent reservoir, and to move a reagent injector cartridge to inject a quantity of reagent into a segment of a chamber; and

a sensor to generate an output signal corresponding to a condition of a fluid sample material within a segment of a chamber.

31. The sample testing system according to claim 30, wherein the coating has a refractive index substantially the same as a refractive index of the segment.

32. A sample testing system comprising, in combination:

a chamber sealing apparatus to form a plurality of seals defining a plurality of fluid-tight segments of the chamber;

a reagent injector cartridge actuator adapted to receive a reagent injector cartridge having at least one needle in fluid communication with a reagent reservoir, and to move a reagent injector cartridge to inject a quantity of reagent into a segment of a chamber;

an incubation chamber to retain a segment of a chamber for a predetermined period of time; and

a sensor to generate an output signal corresponding to a condition of a fluid sample material within a segment of a chamber.

33. The sample testing system according to claim 32, wherein the incubation chamber includes a temperature control device to control the temperature of a segment of a chamber in the incubation chamber.