Methods for attaching substances to surfaces
First Claim
1. A method for immobilizing a substance to a surface, said method comprising:
- (a) combining (i) a surface comprising a linking group consisting of a first portion comprising a hydrocarbon chain, optionally substituted, and a second portion comprising an alkylene oxide or an alkylene imine wherein said alkylene is optionally substituted and wherein one end of said first portion is attached to the surface and one end of said second portion is attached to the other end of said first portion by means of an amine or an oxy functionality and wherein said second portion terminates in an amine or a hydroxy functionality and (ii) the substance to be immobilized, and (b) treating said combination under conditions wherein said substance becomes attached to said surface through the intermediacy of said linking group.
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Accused Products
Abstract
Methods are disclosed for immobilizing a substance to a surface. A surface is employed that comprises a linking group consisting of a first portion comprising a hydrocarbon chain, optionally substituted, and a second portion comprising an alkylene oxide or an alkylene imine wherein the alkylene is optionally substituted. One end of the first portion is attached to the surface and one end of the second portion is attached to the other end of the first portion chain by means of an amine or an oxy functionality. The second portion terminates in an amine or a hydroxy functionality. The surface is reacted with the substance to be immobilized under conditions for attachment of the substance to the surface by means of the linking group. Compositions of matter and reaction systems are also disclosed.
200 Citations
28 Claims
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1. A method for immobilizing a substance to a surface, said method comprising:
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(a) combining (i) a surface comprising a linking group consisting of a first portion comprising a hydrocarbon chain, optionally substituted, and a second portion comprising an alkylene oxide or an alkylene imine wherein said alkylene is optionally substituted and wherein one end of said first portion is attached to the surface and one end of said second portion is attached to the other end of said first portion by means of an amine or an oxy functionality and wherein said second portion terminates in an amine or a hydroxy functionality and (ii) the substance to be immobilized, and (b) treating said combination under conditions wherein said substance becomes attached to said surface through the intermediacy of said linking group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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4. A method according to claim 3 wherein m, n and p are 2, and q is 2 or 4 and wherein said hydrocarbon chain is an alkyl chain of 8 to 12 carbon atoms in length.
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5. A method according to claim 1 wherein said alkyl chain is attached to said surface by means of a silyloxy linkage.
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6. A method according to claim 1 wherein said substance is a ligand or a receptor.
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7. A method according to claim 1 wherein said substance is a nucleoside, a nucleotide or a polynucleotide.
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8. A method according to claim 1 wherein said substance comprises an amine-specific reactive functional group and said alkylene chain or said alkylene imine terminates in an amine group.
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9. A method according to claim 1 wherein said substance comprises an alcohol-specific reactive functional group and said alkylene chain or said alkylene imine terminates in a hydroxy group.
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10. A method according to claim 1 wherein said alkylene imine is a polyiminoalkylene chain represented by the formula:
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11. A composition comprising a surface having attached thereto, at a density of 1 to about 1×
- 107 per square micron, a compound of the formula;
- View Dependent Claims (12, 13, 14, 15, 16, 17)
- 107 per square micron, a compound of the formula;
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18. A combination of compositions comprising:
(a) a composition comprising a surface having attached thereto, at a density of 1 to about 1×
107 per square micron, a compound of the formula;
- View Dependent Claims (19, 20, 21, 22, 23)
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24. A method for immobilizing a substance to a surface, said method comprising:
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(a) combining in a reaction medium (i) said substance, (ii) a first composition comprising a surface having attached thereto, at a density of 1 to about 1×
107 per square micron, a compound of the formula;
- View Dependent Claims (25, 26, 27, 28)
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Specification