Oligonucleotide mediated nucleic acid recombination
First Claim
Patent Images
1. A method of recombining two or more nucleic acids, the method comprising:
- (i.) aligning two or more nucleic acid sequence strings representing the two or more nucleic acids to identify regions of identity and regions of diversity;
(ii.) providing a non-equimolar set of selected oligonucleotides, which set of selected oligonucleotides comprises a plurality of oligonucleotides which correspond in sequence to at least two of the two or more nucleic acids at at least one region of diversity, the oligonucleotides being present in non-equimolar amounts; and
, (iii.) extending the oligonucleotides with a polymerase, thereby producing a plurality of recombinant nucleic acids.
2 Assignments
0 Petitions
Accused Products
Abstract
Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
-
Citations
7 Claims
-
1. A method of recombining two or more nucleic acids, the method comprising:
-
(i.) aligning two or more nucleic acid sequence strings representing the two or more nucleic acids to identify regions of identity and regions of diversity;
(ii.) providing a non-equimolar set of selected oligonucleotides, which set of selected oligonucleotides comprises a plurality of oligonucleotides which correspond in sequence to at least two of the two or more nucleic acids at at least one region of diversity, the oligonucleotides being present in non-equimolar amounts; and
,(iii.) extending the oligonucleotides with a polymerase, thereby producing a plurality of recombinant nucleic acids. - View Dependent Claims (2, 3, 4, 5, 6, 7)
(iv.) selecting the plurality of recombinant nucleic acids for a desired trait or property.
-
-
5. The method of claim 4, further comprising repeating any of steps (i.)-(iv.).
-
6. The method of claim 1, further comprising recombining the recombinant nucleic acid with an additional nucleic acid.
-
7. The method of claim 6, further comprising selecting the resulting further recombined nucleic acid for a desired trait or property.
Specification