Single nucleotide detection using degradation of a fluorescent sequence
First Claim
1. A method for detecting a plurality of single nucleotide polymorphisms (snps) present in a sample of target DNA, said method comprising the steps of:
- combining under primer extension conditions, said target DNA and, for each snp in said target DNA to be detected, a pair of oligonucleotides comprising a primer and a snp-specific snp detection sequence, wherein said primer extension conditions comprise a polymerase having 5′
-3′
exonuclease activity, wherein said primer specifically binds to a first strand of said target DNA and said snp detection sequence also binds to said first strand of said target DNA downstream from said primer in the direction of primer extension and at the site of said snp in said target DNA, and wherein said snp detection sequence comprises (i) an oligonucleotide comprising a base other than the terminal nucleotides that is complementary to one of said plurality of snps in said target DNA and at least one nucleotide linkage resistant to nuclease hydrolysis at a position immediately 3′
of the second nucleotide from the 5′
-end of said oligonucleotide, and (ii) an electrophoretic tag comprising a linker and a fluorophor, wherein said electrophoretic tag is joined to the 5′
-end nucleotide of said oligonucleotide, such that cleavage of the snp-detection sequence at its 5′
end by the 5′
-3′
exonuclease produces a single released fluorescent product composed of the electrophoretic tag and the 5′
-end nucleotide of the snp-detection sequence, wherein each said released fluorescent product produced from a given snp detection sequence has a known, unique electrophoretic mobility with respect to the released fluorescent products produced from all other such snp detection sequences, by virtue of a unique charge/mass ratio associated with the electrophoretic tag;
by said combining, producing said single released fluorescent product for each associated snp present in the sample;
separating said released fluorescent products by electrophoresis; and
identifying each snp present in said sample by the presence of said single released fluorescent product whose electrophoretic mobility is associated with that snp.
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Accused Products
Abstract
Methods and compositions are provided for detecting single nucleotide polymorphisms using a pair of oligonucleotides, a primer and a snp detection sequence, where the snp detection sequence hybridizes to the target DNA downstream from the primer and in the direction of primer extension. The snp detection sequence is characterized by having a nucleotide complementary to the snp and adjacent nucleotide complementary to adjacent nucleotides in the target and an electophoretic tag bonded to the 5′-nucleotide. The pair of oligonucleotides is combined with the target DNA under primer extension conditions, where the polymerase has 5′-3′ exonuclease activity. When the snp is present, the electophoretic tag is released from the snp detection sequence, and can be detected by electrophoresis as indicative of the presence of the snp in the target DNA.
113 Citations
12 Claims
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1. A method for detecting a plurality of single nucleotide polymorphisms (snps) present in a sample of target DNA, said method comprising the steps of:
-
combining under primer extension conditions, said target DNA and, for each snp in said target DNA to be detected, a pair of oligonucleotides comprising a primer and a snp-specific snp detection sequence, wherein said primer extension conditions comprise a polymerase having 5′
-3′
exonuclease activity, wherein said primer specifically binds to a first strand of said target DNA and said snp detection sequence also binds to said first strand of said target DNA downstream from said primer in the direction of primer extension and at the site of said snp in said target DNA, and wherein said snp detection sequence comprises (i) an oligonucleotide comprising a base other than the terminal nucleotides that is complementary to one of said plurality of snps in said target DNA and at least one nucleotide linkage resistant to nuclease hydrolysis at a position immediately 3′
of the second nucleotide from the 5′
-end of said oligonucleotide, and (ii) an electrophoretic tag comprising a linker and a fluorophor, wherein said electrophoretic tag is joined to the 5′
-end nucleotide of said oligonucleotide, such that cleavage of the snp-detection sequence at its 5′
end by the 5′
-3′
exonuclease produces a single released fluorescent product composed of the electrophoretic tag and the 5′
-end nucleotide of the snp-detection sequence, wherein each said released fluorescent product produced from a given snp detection sequence has a known, unique electrophoretic mobility with respect to the released fluorescent products produced from all other such snp detection sequences, by virtue of a unique charge/mass ratio associated with the electrophoretic tag;
by said combining, producing said single released fluorescent product for each associated snp present in the sample;
separating said released fluorescent products by electrophoresis; and
identifying each snp present in said sample by the presence of said single released fluorescent product whose electrophoretic mobility is associated with that snp. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A kit for detecting one or more of a plurality of single nucleotide polymorphisms (snps) that may be present in a sample of target DNA, said kit comprising a plurality of snp detection sequences, wherein each said snp detection sequence comprises:
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(i) an oligonucleotide consisting of at least 12 nucleotides, and containing a nucleotide other than the 5′
or 3′
terminal nucleotides that is complementary to a snp in a target DNA, and at least one nucleotide linkage at positions immediately 3′
of the second nucleotide at the 5′
-end of said oligonucleotide that is resistant to nuclease hydrolysis; and
(ii) a snp-specific electrophoretic tag comprising a linker and a fluorophor, wherein said electrophoretic tag is joined to the 5′
-end nucleotide of said oligonucleotide, and wherein cleavage of the snp-detection sequence between the first and second nucleotides at the 5′
-end of said oligonucleotide produces a single released fluorescent product composed of the electrophoretic tag and the 5′
-end nucleotide of the snp-detection sequence, wherein each said released fluorescent product produced from a given snp detection sequence has a known, unique electrophoretic mobility with respect to the released fluorescent products produced from snp detection sequences specific for other snps, by virtue of a unique charge/mass ratio associated with the electrophoretic tag;
wherein cleavage of a snp detection sequence by a 5′
-to-3′
exonuclease produces, for each snp detection sequence, a single snp-specific fluorescent product containing a snp-specific electrophoretic tag and the 5′
nucleotide of said oliognucleotide.- View Dependent Claims (8, 9, 10, 11, 12)
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Specification