Solid medium and methods for processing a sample of genetic material

• US 6,322,983 B1
• Filed: 08/04/1999
• Issued: 11/27/2001
• Est. Priority Date: 10/05/1988
• Status: Expired due to Fees

• Current Assignee: Whatman PLC (GE Aerospace)
• Original Assignee: Whatman PLC (GE Aerospace)
• Inventors: Burgoyne, Leigh Alexander
• Primary Examiner(s): Meyers, Carla J.
• Assistant Examiner(s): JOHANNSEN, DIANA B
• Application Number:
  US09/368,592
• Time in Patent Office: 846 Days
• Field of Search: 424/486, 424/488, 424/489, 424/490, 424/484, 435/6
• US Class Current: 435/5
• CPC Class Codes: C12N 15/1017 by filtration, e.g. using filters, frits, membranes
• CPC Class Codes: Y10S 435/97 Test strip or test slide

Abstract

A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use of DNA are also disclosed.

Claims

1. A solid medium for processing a sample of genetic material, the solid medium consisting of:

(a) a solid matrix; and

(b) a composition which protects against degradation of genetic material applied to the solid matrix, the composition consisting of;

(i) a protein denaturing agent; and

(ii) an inhibitor of an action of a nuclease.

2. A method for processing a sample of genetic material, the method consisting essentially of:

(a) applying a sample of genetic material to a solid medium wherein the solid medium consists of;

(i) a solid matrix; and

(ii) a composition which protects against degradation of genetic material, the composition being adsorbed or incorporated onto the solid matrix, the composition consisting of a protein denaturing agent and an inhibitor of an action of a nuclease; and

(b) processing the sample of genetic material applied to the solid medium.

3. A method for processing a sample of genetic material, the method consisting essentially of:

(a) applying a sample of genetic material to a solid medium wherein the solid medium comprises;

(i) a solid matrix; and

(ii) a composition which protects against degradation of genetic material, the composition being adsorbed or incorporated onto the solid matrix, the composition comprising a protein denaturing agent, a chelating agent, and uric acid; and

(b) processing the sample of genetic material applied to the solid medium.

4. A solid medium for storing a sample of genetic material, the solid medium consisting of:

(a) a solid matrix; and

(b) a composition which protects against degradation of genetic material adsorbed or incorporated onto the solid matrix, the composition consisting of;

(i) a protein denaturing agent;

(ii) a compound that binds ions which promote degradation of DNA; and

(iii) a buffer.