Systems and methods for handling and manipulating multi-well plates

• US 6,323,035 B1
• Filed: 09/24/1997
• Issued: 11/27/2001
• Est. Priority Date: 09/24/1997
• Status: Expired due to Fees

• Current Assignee: Smithkline Beecham Corporation (GSK plc)
• Original Assignee: Glaxo Wellcome Incorporated (GSK plc)
• Inventors: Kedar, Haim, Brown, Albert William Jr.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): WRIGHT, PATRICIA KATHRYN
• Application Number:
  US08/937,139
• Time in Patent Office: 1,525 Days
• Field of Search: 422/63, 422/65, 422/50, 422/81, 422/68.1, 422/104, 435/288.3, 435/288.4, 436/43, 436/47, 436/49, 414/277, 414/281, 414/807
• US Class Current: 436/43
• CPC Class Codes: G01N 2035/042 moved independently, e.g. by fork manipulator
• CPC Class Codes: G01N 2035/0425 Stacks, magazines or elevators for plates
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]

Abstract

The invention provides exemplary systems, devices and methods for manipulating and handling multi-well plates. In one exemplary embodiment, a system is provided which comprises a robot having a base member and at least one arm. The arm includes a grasping mechanism which is adapted to grasp the plate. Further, the grasping mechanism is configured to receive the plate in a repeatable and known location such that the location of each well relative to the grasping mechanism is known by the robot.

Claims

1. A system for handling a plate having a plurality of wells, the system comprising:

a robot having a base member and at least one arm and a processor controlling the robot, wherein the processor is configured to control movement of the robot to place the plate at predetermined locations at a plurality of laterally spaced apart stations, wherein the arm includes a grasping mechanism which is adapted to grasp the plate from a rack having a plurality of patterned shelves for housing a plurality of plates, wherein the grasping mechanism is movable by the robot along X, Y, and Z axes, and wherein the grasping mechanism includes a frame member with a patterned opening that corresponds in shape to that of the patterned shelves and is configured to receive the plate from the rack in a repeatable and known location said processor configured to, position the frame member below one of the shelves and upwardly moving the frame member so that such one of the shelves passes through the patterned opening such that the location of each well relative to the grasping mechanism is known by the robot;

said plurality of laterally spaced apart stations at known locations relative to the robot, wherein at least one of the stations includes the rack and at least another of the stations includes equipment operable with a two-dimensional array of wells in the plate, including an array of fluid delivery lines to allow fluids, particles or substances to be introduced into the two-dimensional array of wells or removed from the non-adjacent wells while the plate is grasped by the grasping mechanism.

2. A system as in claim 1, wherein the plate has at least 864 wells, and wherein selective ones of the wells of the plate are aligned with the equipment when the plate is placed at the predetermined locations.

3. A system as in claim 1, wherein the arm comprises a shaft, an elbow attached to the shaft, and a linkage pivotally attached to the elbow, and wherein the grasping mechanism is operably attached to the linkage.

4. A system as in claim 3, wherein the shaft is rotatable relative to the base member and is movable in a vertical direction, and wherein the linkage is pivotable relative to the shaft to move the grasping mechanism radially outward from the base member.

5. A system as in claim 1, wherein the frame member has edges which are adapted to frame at least a portion of a periphery of the plate in a repeatable and predictable manner so that the location of the wells of the plate relative to the frame member is known when the plate is received into the frame member;

and a securing mechanism adapted to secure the plate to the frame member when the plate is received within the frame member.

6. A method for handling a plate having a plurality of wells, the method comprising:

providing a robot having a base member and at least one arm, wherein the arm includes a grasping mechanism having a frame member with a patterned opening;

grasping a plate having a plurality of wells from a rack with the grasping mechanism, the rack having a plurality of patterned shelves that correspond in shape to that of the patterned opening for housing a plurality of plates, by positioning the frame member below one of the shelves and upwardly moving the frame member so that such one of the shelves passes through the patterned opening such that the location of each well in the plate relative to the grasping mechanism is known by the robot;

moving the plate with the robot along X, Y and Z axes to a predetermined location at a processing station such that the location of the wells of the plate relative to the station is known; and

introducing fluids into or aspirating fluids from a first two-dimensional array of the wells while the plate is at a first predetermined location and while the plate is being grasped by the grasping mechanism.

7. A method as in claim 6, further comprising moving the plate with the robot to a second predetermined location at the processing station and introducing fluids into or aspirating fluids from a second two-dimensional array of the wells.

8. A method as in claim 6, further comprising moving the plate with the robot to a predetermined location at another processing station, and further comprising introducing a biological substance into the first two-dimensional array of wells while the plate is at the predetermined location.

9. A method as in claim 6, further comprising moving the plate with the robot to an evaluation station, and further comprising imaging the first two-dimensional array of wells with a camera while the plate is at the evaluation station.

10. A method as in claim 9, wherein the first two-dimensional array of wells is aligned with pixels of the camera when the plate is at the evaluation station.

11. A method as in claim 6, wherein the plate has at least 864 wells, wherein the first two-dimensional array of wells comprises 96 wells, and further comprising introducing fluids into or aspirating fluids from the two dimensional array of 96 wells.