Method of making crosslinked polymer matrices in tissue treatment applications
First Claim
1. A method for preparing a negatively charged compound-containing matrix useful for delivery of a negatively charged compound to a mammalian subject, comprising the steps of:
- providing a first synthetic polymer containing nucleophilic groups and a second synthetic polymer containing electrophilic groups;
forming a mixture by mixing the first synthetic polymer and the second synthetic polymer to initiate crosslinking, wherein the first synthetic polymer is present in the mixture in molar excess compared to the second synthetic polymer;
allowing the first synthetic polymer and the second synthetic polymer to continue crosslinking to form a positively charged crosslinked synthetic polymer matrix; and
reacting the matrix with the negatively charged compound.
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Accused Products
Abstract
Crosslinked polymer compositions comprise a first synthetic polymer containing multiple nucleophilic groups covalently bound to a second synthetic polymer containing multiple electrophilic groups. The first synthetic polymer is preferably a synthetic polypeptide or a polyethylene glycol that has been modified to contain multiple nucleophilic groups, such as primary amino (—NH2) or thiol (—SH) groups. The second synthetic polymer may be a hydrophilic or hydrophobic synthetic polymer which contains, or has been derivatized to contain, two or more electrophilic groups, such as succinimidyl groups. The compositions may further comprise other components, such as naturally occurring polysaccharides or proteins (such as glycosaminoglycans or collagen) and/or biologically active agents. Also disclosed are methods for using the crosslinked polymer compositions to effect adhesion between a first surface and a second surface; to effect tissue augmentation; to prevent the formation of surgical adhesions; and to coat a surface of a synthetic implant.
298 Citations
12 Claims
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1. A method for preparing a negatively charged compound-containing matrix useful for delivery of a negatively charged compound to a mammalian subject, comprising the steps of:
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providing a first synthetic polymer containing nucleophilic groups and a second synthetic polymer containing electrophilic groups;
forming a mixture by mixing the first synthetic polymer and the second synthetic polymer to initiate crosslinking, wherein the first synthetic polymer is present in the mixture in molar excess compared to the second synthetic polymer;
allowing the first synthetic polymer and the second synthetic polymer to continue crosslinking to form a positively charged crosslinked synthetic polymer matrix; and
reacting the matrix with the negatively charged compound. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for preparing a positively charged compound-containing matrix useful for delivery of a positively charged compound to a mammalian subject, comprising the steps of:
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providing a first synthetic polymer containing nucleophilic groups and a second synthetic polymer containing electrophilic groups;
forming a mixture by mixing the first synthetic polymer and the second synthetic polymer to initiate crosslinking, wherein the second synthetic polymer is present in the mixture in molar excess compared to the second synthetic polymer;
allowing the first synthetic polymer and the second synthetic polymer to continue crosslinking to form a negatively charged crosslinked synthetic polymer matrix; and
reacting the matrix with the positively charged compound. - View Dependent Claims (8, 9, 10, 11, 12)
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Specification