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Quenching oligonucleotides

  • US 6,323,337 B1
  • Filed: 05/12/2000
  • Issued: 11/27/2001
  • Est. Priority Date: 05/12/2000
  • Status: Expired due to Term
First Claim
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1. A method for decreasing detectable luminescence of nucleic acid stain molecules that are noncovalently associated with an oligonucleotide, said method comprising:

  • a) preparing the oligonucleotide by covalently attaching a quenching moiety to the oligonucleotide, wherein said quenching moiety is essentially nonfluorescent;

    b) combining said oligonucleotide with a plurality of nucleic acid stain molecules such that the nucleic acid stain molecules associate noncovalently with said oligonucleotide;

    wherein said nucleic acid stain molecules normally exhibit a luminescence when associated noncovalently with nucleic acids, and said nucleic acid stain molecules undergo energy transfer to said quenching moiety, such that said luminescence is detectably decreased.

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