Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
First Claim
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1. A protein adsorption resistant surface having the formula:
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Abstract
Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.
179 Citations
28 Claims
- 1. A protein adsorption resistant surface having the formula:
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7. A protein adsorption resistant surface having the formula:
- 8. A protein adsorption resistant surface having the formula:
- 10. A protein adsorption resistant surface having the formula:
- 12. A protein adsorption resistant surface having the formula:
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20. A protein adsorption resistant surface having the formula:
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22. A method of manufacturing a protein adsorption resistant microfluidic device having at least one microchannel therein, said microchannels having a surface, said method comprising:
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(a) derivatizing said surface of said microchannel with a first modifying agent;
(b) monitoring the extent of said derivatizing by;
(i) establishing an electroosmotic flow of a material within said channel;
(ii) measuring a property of said flow to obtain a value; and
,(iii) comparing said value to a reference value for said property, thereby monitoring said extent of said providing. - View Dependent Claims (23, 24, 26)
wherein, n is a number between 1 and 100;
(c) monitoring the extent of said derivatizing by;
(iv) establishing an electroosmotic flow of a material within said channel;
(v) measuring a property of said flow to obtain a value; and
(vi) comparing said value with a reference value for said property, thereby monitoring said extent of said providing.
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26. The method according to claim 22 or 25, wherein said reference value is determined by performing electroosmotic flow in a microfluidic device with a channel having a surface which is not derivatized with a surface modifying reagent.
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25. A method of manufacturing a protein adsorption resistant microfluidic device having at least one microchannel therein, said microchannels having a surface coating comprising n subunits, said method comprising:
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(a) derivatizing said surface of said microchannel with a surface modifying agent;
(b) monitoring the extent of said derivatizing by;
(i) establishing an electroosmotic flow of a material within said channel;
(ii) measuring a property of said flow to obtain a value; and
,(iii) comparing said value to a reference value for said property, thereby monitoring said extent of said providing; and
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(c) repeating steps (a) and (b) n−
1 times.
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Specification