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Enzymatic DNA molecules

  • US 6,326,174 B1
  • Filed: 08/25/1997
  • Issued: 12/04/2001
  • Est. Priority Date: 12/02/1994
  • Status: Expired due to Term
First Claim
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1. A method of selecting a catalytic DNA molecule that cleaves a substrate nucleic acid sequence at a specific site, comprising the following steps:

  • a. obtaining a population of single-stranded DNA molecules;

    b. admixing nucleotide-containing substrate molecules with said population of single-stranded DNA molecules to form an admixture;

    c. maintaining said admixture for a sufficient period of time and under predetermined reaction conditions to allow single-stranded DNA molecules in said population to cause cleavage of said substrate sequences, thereby producing substrate cleavage products;

    d. separating said population of single-stranded DNA molecules from said substrate sequences and substrate cleavage products; and

    e. isolating single-stranded DNA molecules that cleave nucleotide-containing substrate at a specific site from said population.

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