Broad range light detection system

• US 6,326,605 B1
• Filed: 08/18/2000
• Issued: 12/04/2001
• Est. Priority Date: 02/20/1998
• Status: Expired due to Term

• Current Assignee: Molecular Devices
• Original Assignee: LJL Biosystems, Inc. (Danaher Corporation)
• Inventors: Stumbo, David P., Modlin, Douglas N., Stellmacher, Rick V.
• Primary Examiner(s): LE, QUE TAN
• Application Number:
  US09/643,221
• Time in Patent Office: 473 Days
• Field of Search: 250/214.SW, 250/214.R, 250/205, 250/214.1, 250/206, 250/223.R, 250/221, 250/227.22, 250/227.11, 356/73, 327/564, 327/565
• US Class Current: 250/214.0SW
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6445 Measuring fluorescence polarisation
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

A broad-range light-detection system. In some embodiments, the system includes apparatus and methods for detecting light with high accuracy over a broad range of intensities. In other embodiments, the system includes apparatus and methods for automatically scaling the detection range to improve detection based on the intensity of the detected light. In yet other embodiments, the system includes apparatus and methods for detecting light with increased speed, particularly in applications involving analysis of successive samples.

Claims

1. A system for determining the amount of light received by a detector during a sampling period, where the detector produces an output which is a function of light being received, the system comprising:

a first integration device operatively connected to the output of the detector and adapted to generate a first integration signal during the sampling period, the first integration signal being proportional to the amount of light received by the detector during the sampling period so long as the amount of light received during the sampling period is less than a first predetermined amount;

a second integration device operatively connected to the output of the detector and adapted to generate a second integration signal during the sampling period, the second integration signal being proportional to the amount of light received by the detector during the sampling period so long as the amount of light received during the sampling period is less than a second predetermined amount, wherein the second predetermined amount is at least several times greater than the first predetermined amount; and

a range selection system connected to the first and second integration devices and configured to automatically select one of the integration signals to use in determining the amount of light received by the detector based upon the amount of light received during the sampling period as represented by the integration signals.

2. The system of claim 1, wherein the range selection system selects between the integrations signals based, at least in part, on whether the first integration signal exceeded the first predetermined amount during the sample period.

3. The system of claim 1, wherein the first integration device discretely counts pulses from the output of the detector corresponding to photons received by the detector.

4. The system of claim 1, wherein the first integration device carries out an analog integration corresponding to the output of the detector to generate the first integration signal.

5. The system of claim 1, further including a third integration device operatively connected to the output of the detector and adapted to generate a third integration signal during the sampling period, the third integration signal being proportional to the amount of light received by the detector during the sampling period so long as the amount of light received during the sampling period is less than a third predetermined amount, wherein the third predetermined amount is as least several times greater than the second predetermined amount.

6. The system of claim 1, further including a timer associated with the first integration device, the timer being configured to measure the time required for the first integration signal to reach a first threshold value.

7. A method of measuring light intensity from a source, comprising:

detecting photons incident on a detector from the source;

generating a first integration signal proportional to the cumulative number of photons incident on the detector during a sampling period so long as the cumulative number of photons is less than a first range value;

simultaneously with generating a first integration value, generating a second integration signal proportional to the number of photons incident on the detector during the sampling period so long as the cumulative number of photons is less than a second range value, where the second range value is at least an order of magnitude larger than the first range value;

selecting one of the integration signals to use in computing a detected light intensity based on whether the cumulative number of photons incident on the detector during the sampling period exceeded the first range value; and

computing a detected light intensity using the selected integration signal.

8. The method of claim 7, further comprising simultaneously with generating first and second integration values, generating a third integration signal proportional to the number of photons incident on the detector during the sampling period so long as the cumulative number of photons is less than a third range value, where the third range value is at least an order of magnitude larger than the second range value.