Target analyte sensors utilizing Microspheres
First Claim
1. A composition comprising:
- a) a substrate with a patterned surface comprising discrete sites, wherein said sites comprise wells and wherein said substrate is a fiber optic bundle or array comprising individual fibers, and said wells are at a first terminal end of said bundle or array; and
b) a population of microspheres randomly distributed on said sites, said population comprising at least a first and a second subpopulation.
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Accused Products
Abstract
A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents may be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modified substrates may be employed which provide either discrete or non-discrete sites for accommodating the microspheres in either random or patterned distributions. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrate to produce a high density array. The disclosed system and method have utility for detecting target analytes and screening large libraries of bioactive agents.
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Citations
39 Claims
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1. A composition comprising:
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a) a substrate with a patterned surface comprising discrete sites, wherein said sites comprise wells and wherein said substrate is a fiber optic bundle or array comprising individual fibers, and said wells are at a first terminal end of said bundle or array; and
b) a population of microspheres randomly distributed on said sites, said population comprising at least a first and a second subpopulation. - View Dependent Claims (2, 3, 4, 9, 10, 11, 12, 13, 14, 15, 16)
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5. A composition comprising:
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a) a substrate with a patterned surface comprising discrete sites at density of at least 100 sites per 1 mm2; and
b) a population of microspheres randomly distributed on said sites, said population comprising at least a first and a second subpopulation, wherein said microspheres comprise a bioactive agent. - View Dependent Claims (6, 7, 8)
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17. A method of determining the presence of at least a first and second target analyte in a sample comprising:
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a) contacting said sample with a composition comprising;
i) a substrate with a patterned surface comprising discrete sites; and
ii) a population of microspheres comprising at least a first and a second subpopulation, wherein said first subpopulation comprises a first bioactive agent and said second subpopulation comprises a second bioactive agent, wherein said microspheres are randomly distributed on said surface such that said discrete sites contain microspheres; and
b) determining the presence of said first and second target analyte. - View Dependent Claims (18, 19, 20, 21)
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22. A method of making a composition comprising:
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a) providing a patterned surface comprising individual sites on a substrate;
b) randomly distributing microspheres on said surface such that said individual sites contain microspheres, wherein said microspheres comprise at least a first and a second subpopulation comprising;
i) a first and second bioactive agent, respectively; and
ii) a first and second optical signature, respectively;
c) detecting said first and second optical signatures while said microspheres are distributed on said surface; and
d) correlating the location of at least one individual site on the array with the bioactive agent at that particular site. - View Dependent Claims (23, 24, 25, 26)
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27. A composition comprising:
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a) a substrate with a surface comprising discrete sites at a density of at least 100 discrete sites per 1 mm2, said discrete sites comprising wells; and
b) a population of microspheres randomly distributed in said wells, said population comprising at least a first and a second subpopulation, said microspheres comprising a bioactive agent. - View Dependent Claims (28, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
c) a sample comprising at least first and second target analytes that will bind said bioactive agents of said first and second subpopulations.
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30. The composition according to claim 4, 5, 27 or 29, wherein said density is at least 500 discrete sites per 1 mm2.
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31. The composition according to claim 4, 5, 27 or 29, wherein said density is at least 1000 discrete sites per 1 mm2.
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32. The composition according to claim 4, 5, 27 or 29, wherein said density is at least 5000 discrete sites per 1 mm2.
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33. The composition according to claim 4, 5, 27 or 29, wherein said density is at least 10,000 discrete sites per 1 mm2.
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34. The composition according to claim 4, 5, 27 or 29, wherein said density is at least 50,000 discrete sites per 1 mm2.
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35. The composition according to claim 5, 27 or 29, wherein said discrete sites are wells.
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36. The composition according to claim 35, wherein microspheres are randomly dispersed in said wells.
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37. The composition according to claim 5, 27, or 29, wherein said substrate is a fiber optic bundle.
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38. The composition according to claim 27 or 29, wherein said substrate is selected from the group consisting of glass and plastic.
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39. A composition according to claim 5, 27 or 29, wherein said bioactive agent comprises a nucleic acid.
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29. A composition comprising:
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a) a substrate with a patterned surface comprising discrete sites, said substrate comprising discrete sites at a density of at least 100 discrete sites per 1 mm2; and
b) a population of microspheres, randomly distributed on said sites, wherein each microsphere comprises a bioactive agent.
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Specification