Very large scale immobilized polymer synthesis
First Claim
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1. A method of synthesizing a plurality of different polymers on a surface of a substrate, comprising:
- (a) providing a substrate having a surface bearing multiple copies of a protective group removable on exposure to an electric field or electric current;
(b) applying the electric field or electric current to the substrate to remove a protective group from a first known location on the surface of the substrate;
c) exposing the surface of the substrate to a first protected monomer bearing a protective group removable on exposure to an electric field or electric current, whereby the protected monomer attaches to the first known location;
(d) applying the electric field or electric current to the substrate to remove a protective group from a second known location on the surface of the substrate;
(e) exposing the surface of the substrate to a second protective monomer bearing a protective group removable on exposure to an electric field or electric current, whereby the second protected monomer attached to the second known location;
(f) repeating (b)-(e) while controlling the known locations on the surface of the substrate to synthesize a plurality of different polymers at known locations on the surface of a substrate.
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Abstract
A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, is provided to the substrate to bind at the selected areas. The process is repeated using a variety of monomers such as amino acids until sequences of a desired length are obtained. Detection methods and apparatus are also disclosed.
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Citations
10 Claims
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1. A method of synthesizing a plurality of different polymers on a surface of a substrate, comprising:
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(a) providing a substrate having a surface bearing multiple copies of a protective group removable on exposure to an electric field or electric current;
(b) applying the electric field or electric current to the substrate to remove a protective group from a first known location on the surface of the substrate;
c) exposing the surface of the substrate to a first protected monomer bearing a protective group removable on exposure to an electric field or electric current, whereby the protected monomer attaches to the first known location;
(d) applying the electric field or electric current to the substrate to remove a protective group from a second known location on the surface of the substrate;
(e) exposing the surface of the substrate to a second protective monomer bearing a protective group removable on exposure to an electric field or electric current, whereby the second protected monomer attached to the second known location;
(f) repeating (b)-(e) while controlling the known locations on the surface of the substrate to synthesize a plurality of different polymers at known locations on the surface of a substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification