Reverse-root-canal method for extracting aDNA
First Claim
1. A nondestructive method for extracting nucleic acid-rich dentin from teeth comprisinga) obtaining teeth to be studied b) generating a canal beginning at the apex, wherein the canal is generated with endodontic files beginning with a small endodontic file followed by larger endodontic files until a canal is generated directly beneath the enameled crown of the tooth c) collecting dentin from the canal d) extracting nucleic acid from the dentin.
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Abstract
Teeth, the hardest substance in the human body, are frequently all that remain of a mortuary population from which direct human presence can be gleaned. As such their morphology is invaluable to physical anthropologists and investigators in allied disciplines. Methods currently used for purposes of extracting DNA from dental remains—e.g. bone-milling, crushing, and sectioning—result in total destruction of the teeth. This paper introduces the Reverse-Root-Canal, a protocol by which DNA of molecular weight higher than that obtainable through traditional destructive means, can be obtained from ancient dental remains without harm to the morphologically informative crown and roots.
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1 Claim
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1. A nondestructive method for extracting nucleic acid-rich dentin from teeth comprising
a) obtaining teeth to be studied b) generating a canal beginning at the apex, wherein the canal is generated with endodontic files beginning with a small endodontic file followed by larger endodontic files until a canal is generated directly beneath the enameled crown of the tooth c) collecting dentin from the canal d) extracting nucleic acid from the dentin.
Specification
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Current AssigneeJanice Cori Cobb
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Original AssigneeJanice Cori Cobb
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InventorsCobb, Janice Cori
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Primary Examiner(s)Arthur, Lisa B.
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Assistant Examiner(s)GOLDBERG, JEANINE ANNE
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Application NumberUS09/520,042Time in Patent Office644 DaysField of Search435/6, 433/102, 433/224, 433/165US Class Current435/6.11CPC Class CodesC12Q 1/6806 Preparing nucleic acids for...
