×

Bacterial multi-hybrid system and applications thereof

  • US 6,333,154 B1
  • Filed: 12/01/1998
  • Issued: 12/25/2001
  • Est. Priority Date: 12/04/1997
  • Status: Expired due to Term
First Claim
Patent Images

1. A bacterial multi-hybrid signal amplification system comprising:

  • (a) a first chimeric polypeptide comprising a first fragment of an enzyme chosen from adenylate cyclase and guanylate cyclase;

    (b) a second chimeric polypeptide comprising a second fragment of an enzyme chosen from adenylate cyclase and guanylate cyclase, or a modulating substance capable of activating adenylate cyclase or guanylate cyclase, and (c) a signal molecule precursor, wherein the first fragment is fused to a molecule of interest, and the second fragment or the modulating substance is fused to a target ligand, and wherein the activity of the enzyme is restored by the in vivo interaction between the molecule of interest and the target ligand and wherein a signal molecule is generated by the restored enzyme activity; and

    wherein the in vivo interaction occurs in a bacterial cell.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×