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Method of forming polysaccharide sponges for cell culture and transplantation

  • US 6,334,968 B1
  • Filed: 10/13/1999
  • Issued: 01/01/2002
  • Est. Priority Date: 05/22/1996
  • Status: Expired due to Term
First Claim
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1. A process for producing a polysaccharide sponge characterized by having:

  • (i) an average pore size in the range between about 10 μ

    m to about 300 μ

    m;

    (ii) an average distance between the pores being the wall thickness of the pores in the range between about 5 μ

    m to about 270 μ

    m; and

    (iii) an E-modului of elasticity being a measure of the rigidity of the sponge in the range of about 50 kPa to about 500 kPa, the process comprising;

    (a) providing a polysaccharide solution containing about 1% to about 3% w/v polysaccharide in water;

    (b) optionally diluting said polysaccharide solution with additional water to obtain a final solution having about 0.5% to about 2% w/v polysaccharide, and subjecting said solution of (a) to gelation, to obtain a polysaccharide gel;

    (c) freezing the gel of (b); and

    (d) drying the frozen gel of (c) to obtain a polysaccharide sponge.

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