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Automated process for isolating and amplifying a target nucleic acid sequence

  • US 6,335,166 B1
  • Filed: 04/30/1999
  • Issued: 01/01/2002
  • Est. Priority Date: 05/01/1998
  • Status: Expired due to Term
First Claim
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1. An automated process for isolating and amplifying a target nucleic acid sequence that may be present in a fluid sample contained in a reaction receptacle, said process comprising:

  • a) separating the target sequence from non-target nucleic acid that may be present in the fluid sample in a separation station;

    b) transporting the reaction receptacle containing the separated target sequence, if present in the fluid sample, from the separation station to an amplifying incubation station comprising one or more incubators, each said incubator of said amplifying incubation station defining a temperature-controlled chamber; and

    c) incubating the contents of the reaction receptacle, to which one or more amplification reagents have been provided, in said amplifying incubation station for a period of time and under conditions sufficient to permit the target sequence to be amplified, wherein each of steps a)-c) is automated.

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