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De novo or “universal” sequencing array

  • US 6,337,188 B1
  • Filed: 08/25/2000
  • Issued: 01/08/2002
  • Est. Priority Date: 11/21/1997
  • Status: Expired due to Fees
First Claim
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1. A method of sequencing a template nucleic acid molecule on a combinatorial array comprising the steps:

  • A) immobilizing a sequencing reagent to a solid surface, wherein said sequencing reagent comprises;

    i) a capture moiety which can form a stable complex with a capture region of a template nucleic acid molecule;

    ii) a spacer region; and

    iii) a sequence specific hybridizing region, wherein said sequence specific hybridizing region comprises 4-8 bases, which are bound to the spacer region and which can hybridize to a complementary region on the template nucleic acid molecule;

    B) hybridizing said capture region of the template nucleic acid molecule to the capture moiety of the sequencing reagent;

    C) hybridizing said sequence specific hybridizing region to said complementary region on the template nucleic acid molecule thereby forming a hybridized sequence specific region;

    D) extending said hybridized sequence specific region by a polymerase with a chain terminating nucleotide thereby forming an extended sequence; and

    E) determining the identity of said extended sequence.

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