Microfluidic devices for electrophoretic analysis of materials
DCFirst Claim
1. A method of separating components of a subject material in a microfluidic system, the method comprising:
- flowing a first fluid comprising the subject material through a first microchannel to a separation channel by applying pressure to the fluid wherein the pressure is applied to the fluid by a pressure source external to the first microchannel; and
applying an electrophoretic force to the first fluid comprising the subject material in the separation channel, thereby separating the subject material into sample components.
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Abstract
The present invention provides a microfluidic system for fast, accurate and low cost electrophoretic analysis or materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light from periodically spaced regions along a channel in the microfluidic system are received by a photodetector. The intensity of light received by the photodetector is modulated by the movement of species bands through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band is determined and the identification of the species is made by its electrophoretic mobility in the channel.
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Citations
22 Claims
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1. A method of separating components of a subject material in a microfluidic system, the method comprising:
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flowing a first fluid comprising the subject material through a first microchannel to a separation channel by applying pressure to the fluid wherein the pressure is applied to the fluid by a pressure source external to the first microchannel; and
applying an electrophoretic force to the first fluid comprising the subject material in the separation channel, thereby separating the subject material into sample components. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A device for separating components of a fluid, the device comprising:
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a microfluidic device comprising a body structure comprising at least first and second intersecting microchannels fabricated therein;
a pressure source fluidly coupled to the first microchannel; and
,a voltage or current controller operably coupled to the second microchannel. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification