Isolated nucleic acid molecules which encode tumor rejection antigens found in dage

• US 6,339,149 B1
• Filed: 08/11/1998
• Issued: 01/15/2002
• Est. Priority Date: 09/30/1994
• Status: Expired due to Term

• Current Assignee: Ludwig Institute for Cancer Research, Ltd.
• Original Assignee: Ludwig Institute for Cancer Research, Ltd.
• Inventors: Ikeda, Hideyuki, Boon-Falleur, Thierry, Coulie, Pierre
• Primary Examiner(s): Huff, Sheela
• Assistant Examiner(s): DENT, ALANA HARRIS
• Application Number:
  US09/131,831
• Time in Patent Office: 1,253 Days
• Field of Search: 435/7.1, 530/350, 530/323, 530/325, 530/326, 530/300, 530/389, 424/184, 424/1, 424/185.1, 536/118.7, 536/22.1, 536/23.1
• US Class Current: 536/23.5
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: C07K 14/4748 Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: Y10S 530/828 Cancer

Abstract

A new family of tumor rejection antigen precursors, and the nucleic acid molecules which code for them, are disclosed. These tumor rejection antigen precursors are referred to as DAGE tumor rejection antigen precursors, and the nucleic acid molecules which code for them are referred to as GAGE coding molecules. Various diagnostic and therapeutic uses of the coding sequences and the tumor rejection antigens, and their precursor molecules are described.

Claims

1. An isolated nucleic acid molecule which consists of a nucleotide sequence that encodes a tumor rejection antigen wherein said tumor rejection antigen consists of an amino acid sequence found in SEQ. ID NO:

17.

2. The isolated nucleic acid molecule of claim 1, which encodes a tumor rejection antigen which complexes with HLA-A24 molecules.

3. The isolated nucleic acid molecule of claim 1, which encodes a tumor rejection antigen which complexes with an HLA-A2, HLA-A3, HLA-A11, HLA-B7, HLA-B3, HLA-B44, or HLA-Cw*1601 molecule.

4. The isolated nucleic acid molecule of claim 1, which encodes tumor rejection antigen consisting of an amino acid sequence defined by amino acids 28-36, 40-48, 48-56, 80-88, 118-126, 150-158, 156-164, 184-192, 195-203, 198-206, 204-212, 254-262, 355-364, 375-383 or 447-455 of SEQ ID NO:

17.

5. The isolated nucleic acid molecule of claim 4, which encodes the amino acid sequence set forth in SEQ ID NO:

6 or SEQ ID NO;

8.