Fatty acid desaturases and mutant sequences thereof

• US 6,342,658 B1
• Filed: 07/16/1999
• Issued: 01/29/2002
• Est. Priority Date: 12/14/1995
• Status: Expired due to Term

• Current Assignee: Cargill, Inc. (Agricultural Services Division)
• Original Assignee: Cargill, Inc. (Agricultural Services Division)
• Inventors: DeBonte, Lorin R., Shorrosh, Basil S.
• Primary Examiner(s): Benzion, Gary
• Application Number:
  US09/354,231
• Time in Patent Office: 928 Days
• Field of Search: 800/281, 800/284, 800/306, 536/24.7, 536/23.6
• US Class Current: 800/306
• CPC Class Codes: C07C 53/126 Acids containing more than four carbon atoms
• CPC Class Codes: C07C 57/02 with only carbon-to-carbon double bonds as unsaturation
• CPC Class Codes: C07C 57/03 Monocarboxylic acids
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)

Abstract

Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

Claims

1. An isolated nucleic acid fragment encoding a polypeptide having the amino acid sequence of SEQ ID NO:

18.

2. An isolated nucleic acid fragment, wherein said nucleic acid fragment is selected from the group consisting of:

a) SEQ ID NO;

17;

b) an RNA comprising the nucleotide sequence of SEQ ID NO;

17, wherein uracil replaces thymine;

c) a nucleic acid fragment having a nucleic acid sequence complementary to a) or b); and

d) a nucleic acid fragment of a), b), or c), that is at least 10 nucleotides in length and that hybridizes under stringent conditions to genomic DNA encoding the mutation in the polypeptide of SEQ ID NO;

18.

3. The nucleic acid fragment of claim 2, wherein said fragment has the nucleotide sequence of SEQ ID NO:

17.

4. An isolated polypeptide having the amino acid sequence of SEQ ID NO:

18.

5. A canola seed designated Q4275 and represented by ATCC accession number 97569.

6. Progeny of the seed of claim 5, said progeny having two mutant delta-12 fatty acid desaturases that are present in seed represented by ATCC accession number 97569.

7. Progeny of the seed of claim 5, said progeny having a mutant F-form of delta-12 fatty acid desaturase that is present in seed represented by ATCC accession number 97569.