Dual pathlength system for light absorbance detection
First Claim
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1. An absorbance detector apparatus, comprising:
- a flow cell having a chamber receiving a sample, said chamber being configured to provide at least a first light path through said sample and a second light path through said sample, said first light path being longer than said second light path;
a light source directing light into said flow cell and into said at least said first light path through said sample and said second light path through said sample; and
a single photo detector or single photodetector array receiving light exiting from said at least said first light path through said sample and said second light path through said sample;
wherein concentration of the sample relates to the measured absorbance by the equation set forth below;
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Abstract
A flow cell for absorbance detection with at least two different optical path lengths. It increases the range of analyte concentrations which can be measured compared with a conventional single path flow cell. Light from the two paths is combined onto the same photodetector. Calibration with known samples allows analyte concentrations to be measured. The dual or multi-path length flow cell may be used in equipment designed for single path flow cells.
50 Citations
27 Claims
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1. An absorbance detector apparatus, comprising:
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a flow cell having a chamber receiving a sample, said chamber being configured to provide at least a first light path through said sample and a second light path through said sample, said first light path being longer than said second light path;
a light source directing light into said flow cell and into said at least said first light path through said sample and said second light path through said sample; and
a single photo detector or single photodetector array receiving light exiting from said at least said first light path through said sample and said second light path through said sample;
wherein concentration of the sample relates to the measured absorbance by the equation set forth below;
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A flow cell, comprising:
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a chamber receiving a sample;
a light transparent tube disposed interior to said chamber; and
at least one of a first light guiding material disposed adjacent to an interior surface of said chamber and a second light guiding material disposed adjacent to an interior surface of said light transparent tube, said light transparent tube is dimensioned with respect to said chamber to form at least a first light path through said sample and a second light path through said sample, said first light path being longer than said second light path;
wherein concentration of the sample relates to the measured absorbance by the equation set forth below;
- View Dependent Claims (15, 16, 17, 18, 19, 20)
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21. A method of forming a flow cell, said method comprising the steps of:
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providing a chamber configured for receiving a sample;
dimensioning a light transparent lube with Respect to said chamber to form at least a first light path through said sample and a second light path through said sample, said first light path being longer than said second light path; and
disposing said light transparent tube interior to said chamber;
wherein concentration of the sample relates to the measured absorbance by the equation set forth below;
- View Dependent Claims (22, 23, 24, 25, 26)
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27. An absorbance detector apparatus, comprising:
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flow cell means for receiving a sample and for providing at least a first light path through said sample and a second light path through said sample, said first light path being longer than said second light path;
light source means for directing light into said at least said first light path through said sample and said second light path through said sample; and
a single detection means receiving light exiting from said at least said first light path through said sample and said second light path through said sample;
wherein concentration of the sample relates to the measured absorbance by the equation set forth below;
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Specification