×

Methods for analysis and sorting of polynucleotides

  • US 6,344,325 B1
  • Filed: 02/08/2000
  • Issued: 02/05/2002
  • Est. Priority Date: 09/25/1996
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of isolating polynucleotide molecules based on size, which method comprises:

  • (a) flowing a stream of a solution containing reporter-labeled polynucleotide molecules through an analysis unit, wherein the analysis unit is microfabricated onto the substrate of a chip and comprises a main channel having (i) a polynucleotide sample inlet, (ii) a detection region downstream of the sample inlet, and (iii) a branch point discrimination region adjacent to and downstream of the detection region, wherein on average one polynucleotide molecule at a time is placed within the detection region;

    (b) determining the size of each polynucleotide molecule as it passes through the detection region by measuring the level of said reporter;

    (c) directing polynucleotide molecules in the flow that have a selected size into a branch channel originating at the branch point discrimination region; and

    (d) collecting polynucleotides directed into the branch channel.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×