Methods for analysis and sorting of polynucleotides

• US 6,344,325 B1
• Filed: 02/08/2000
• Issued: 02/05/2002
• Est. Priority Date: 09/25/1996
• Status: Expired due to Term

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Quake, Stephen, Volkmuth, Wayne D.
• Primary Examiner(s): NOLAND, THOMAS
• Application Number:
  US09/499,943
• Time in Patent Office: 728 Days
• Field of Search: 435/6, 435/287.3, 435/288.7, 435/286.5, 435/286.6, 435/286.1, 435/287.1, 738/658
• US Class Current: 435/6.16
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0652 Sorting or classification of particles or molecules
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0484 Cantilevers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10S 366/03 Micromixers: variable geometry from the pathway influences mixing/agitation of non-laminar fluid flow

Abstract

The invention relates to a microfabricated device and methods of using the device for analyzing and sorting individual polynucleotides, e.g., by size according to an optical signal measured within a detection region of the device. An optical signal such as fluorescence from a reporter molecule associated with the polynucleotide molecules can be used to determine polynucleotide size or to direct selected polynucleotides into one or more selected branch channels of the device.

Claims

1. A method of isolating polynucleotide molecules based on size, which method comprises:

(a) flowing a stream of a solution containing reporter-labeled polynucleotide molecules through an analysis unit,

wherein the analysis unit is microfabricated onto the substrate of a chip and comprises a main channel having (i) a polynucleotide sample inlet, (ii) a detection region downstream of the sample inlet, and (iii) a branch point discrimination region adjacent to and downstream of the detection region, wherein on average one polynucleotide molecule at a time is placed within the detection region;

(b) determining the size of each polynucleotide molecule as it passes through the detection region by measuring the level of said reporter;

(c) directing polynucleotide molecules in the flow that have a selected size into a branch channel originating at the branch point discrimination region; and

(d) collecting polynucleotides directed into the branch channel.

2. The method of claim 1, wherein the concentration of polynucleotide molecules in the solution is between about 100 fM and about 100 pM.

3. The method of claim 1, wherein the detection region has a volume between about 1 fl and about 1 pl.

4. The method of claim 1, wherein the size of each polynucleotide molecule is determined by quantitating an optical signal from an optical reporter associated with the polynucleotide molecules.

5. The method of claim 4, wherein the optical signal is a fluorescence signal.

6. The method of claim 1, wherein the selected size is between about 100 bp and about 10 mb.

7. The method of claim 1, wherein the selected size is between about 100 bp and about 100 kb.

8. The method of claim 1, wherein the selected size is between about 500 bp and about 50 kb.

9. The method of claim 1, wherein a transparent cover slip is bonded to the substrate of the chip and covers channels of the analysis unit.

10. The method of claim 1, wherein a microscope is associated with the detection region so that the microscope measures the level of a reporter associated with the polynucleotide molecules.

11. The method of claim 10, wherein the microscope is a fluorescence microscope and excites a fluorescent reporter.

12. The method of claim 10, wherein the microscope comprises a high numerical aperture objective.

13. The method of claim 10, wherein the microscope objective has a numerical aperture of about 1.4.

14. The method of claim 1, wherein a laser excites a reporter associated with the polynucleotide molecules and an integrated photodiode is associated with the detection region that measures the level of reporter.

15. The method of claim 1, wherein the polynucleotide molecules are associated with an optically detectable reporter.

16. The method of claim 15, wherein the reporter comprises at least one molecule selected from the group consisting of fluorescent molecules and chemiluminescent molecules.

17. The method of claim 15, wherein the reporter comprises at least one fluorescent molecule selected from the group consisting of POPO, BOBO, YOYO and TOTO.

18. The method of claim 1, wherein the flow of polynucleotides is directed by a pair of electrodes that apply an electric field across the discrimination region and into a branch channel.

19. The method of claim 1, wherein the flow of polynucleotide molecules is directed by adjusting the pressure at the outlet of each branch channel to allow or curtain flow through the channel.

20. The method of claim 1, wherein the detection region contains an optical detector having a short optical path length.

21. The method of claim 1, wherein the analysis unit further comprises a sample solution reservoir.

22. The method of claim 1, wherein a microprocessor is in communication with at least one of:

a detector that measures the level of the reporter; and

a flow control that directs the flow of polynucleotide molecules.

23. The method of claim 1, wherein the flow of polynucleotide molecules is directed by at least one pump and at least one valve.