Method and apparatus for controlling a stream of liquid test packages in a capsule chemistry analysis system

• US 6,348,354 B1
• Filed: 07/06/1998
• Issued: 02/19/2002
• Est. Priority Date: 07/06/1998
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Bayer Corporation (Bayer AG)
• Inventors: Gherson, Paul, Lightbody, David, Adolfsen, Robert
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Gordon, Brian R.
• Application Number:
  US09/111,162
• Time in Patent Office: 1,324 Days
• Field of Search: 436/43, 436/50, 436/52, 436/53, 436/54, 436/180, 422/63, 422/81, 422/82, 422/103, 422/105, 422/108
• US Class Current: 436/53
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A method and apparatus for controlling a stream of liquid and air segments wherein the liquid and air segments are selectively aspirated into a first fluid conduit in a plurality of cycles, each cycle beginning with the aspiration of a first air segment and ending with the aspiration of a final air segment. The liquid and air segments are then transferred from the first fluid conduit to a second fluid conduit. The volume of the final air segment of each cycle is then adjusted after the final air segment has moved into the second fluid conduit. Next, the liquid segments and the air segments of each cycle are transferred from the second fluid conduit to a third fluid conduit. The volume of the first air segment of each cycle is then adjusted after the first air segment has moved into the third fluid conduit.

Claims

1. An apparatus for controlling a stream of liquid packages, each liquid package comprising a plurality of liquid and air segments, the apparatus comprising:

a first and second fluid conduit each being internally coated with an isolation liquid;

a probe for selectively aspirating liquid segments and air segments into the first fluid conduit in a plurality of cycles, each cycle ending with the aspiration of a final liquid segment and a final air segment in that order said liquid segments each occupying a length of said first and second fluid conduits wherein the length occupied by each liquid segment is dependent upon the surface tension of the liquid comprising the segment,

a valve coupled to said first fluid conduit and said second fluid conduit, said valve being adapted to be actuated between a first position in which said second fluid conduit is coupled to said first fluid conduit and a second position in which said second fluid conduit is not coupled to said first fluid conduit;

an air and liquid interface detector positioned along said second fluid conduit a fixed distance from said valve, said fixed distance corresponding to a predetermined fixed volume within said second fluid conduit; and

a means for stopping the aspiration of the final air segment in a current cycle when said air and liquid interface detector detects an interface between an air segment and a liquid segment of a previous cycle which have moved into said second fluid conduit after the final liquid segment of said previous cycle has fully entered said second fluid conduit and for actuating said valve from said first position to said second position at a time subsequent to said detection;

wherein stopping the aspiration of the final air segment in a current cycle controls the length of the liquid segments in the previous and current cycle ensuring that the liquid and air segments of the previous cycle are at a desired distance from the valve before the valve is actuated, and

wherein each of said cycles begins with the aspiration of a first air segment and a first liquid segment in that order, the apparatus further comprising;

a third fluid conduit being internally coated with an isolation liquid into which the liquid and air segments aspirated in each of said cycles are inserted and in which the stream of liquid packages is repeatedly bi-directionally flowed in a forward and a reverse direction, said third fluid conduit being coupled to said valve such that when said valve is in said second position said third fluid conduit is coupled to said second fluid conduit;

a second air and liquid interface detector positioned along said third fluid conduit adjacent said valve; and

means for stopping said flow of said stream of liquid packages in said reverse direction at a point in time when said second air and liquid interface detector detects an interface between the first air segment and the first liquid segment most recently inserted into said third fluid conduit adjusted by a delay, said delay being normalized around a predetermined nominal center point delay according to a feedback loop.

2. An apparatus according to claim 1, wherein said valve comprises a shear valve.

3. An apparatus according to claim 1, wherein said air and liquid interface detector comprises a bubble detector.

4. An apparatus according to claim 1, said feedback loop being based upon a time differential TD equal to T₂−

T₁, wherein T₁ is a time at which a first particular liquid segment of a particular liquid package reaches a reference during said flow of said stream in said forward direction and T₂ is a time at which a second particular liquid segment of said particular liquid package reaches said reference during said flow of said stream in said reverse direction.

5. An apparatus according to claim 4, said delay being calculated according to the following formula:

6. An apparatus according to claim 4, wherein said reference point comprises a third air and liquid interface detector positioned along said third fluid conduit.

7. An apparatus according to claim 1, wherein the liquid and air segments aspirated in each of said cycles and inserted into said third fluid conduit each comprise a liquid package having a length, wherein said length is measured for a plurality of said liquid packages during said flow of said stream in either said forward or said reverse direction and wherein an average length is calculated using a plurality of said measured lengths, said feedback loop being based upon a difference between a target liquid package length and said average length.

8. An apparatus according to claim 7, said delay being calculated according to the following formula:

9. A method for controlling a stream of liquid packages, each liquid package comprising a plurality of liquid and air segments, the method comprising:

selectively aspirating liquid segments and air segments into a first fluid conduit in a plurality of cycles, each liquid segment occupying a length of the first and second fluid conduits which length is dependent upon the surface tension of the liquid in each segment,

each cycle ending with the aspiration of a final liquid segment and a final air segment in that order, said first fluid conduit being coupled to a valve, said valve being coupled to a second fluid conduit, wherein said valve is adapted to be actuated between a first position in which said first fluid conduit is coupled to said second fluid conduit and a second position in which said first fluid conduit is not coupled to said second fluid conduit;

detecting an interface between an air segment and a liquid segment of a previous cycle which have moved into said second fluid conduit at a position along said second fluid conduit which is a fixed distance form said valve after the final liquid segment of said previous cycle has fully entered said second fluid conduit, said fixed distance corresponding to a predetermined fixed volume within said second fluid conduit; and

stopping the aspiration of the final air segment in a current cycle when said interface has been detected so as to ensure that the liquid and air segment of a previous cycle have moved the fixed distance from the valve;

and actuating said valve from said first position to said second position at a time subsequent to said detection,

wherein each of said cycles begins with the aspiration of a first air segment and a first liquid segment in that order, the method further comprising;

inserting the liquid segments and the air segments aspirated in each of said cycles into a third fluid conduit such that for each of said cycles the first liquid segment and the first air segment are the next-to-last and the last liquid and air segment, respectively, inserted;

flowing said stream of liquid packages in a forward direction and a reverse direction in said third fluid conduit said third fluid conduit being coupled to said valve such that when said valve is in said second position said third fluid conduit is coupled to said second fluid conduit;

detecting an interface between the first air segment and the first liquid segment most recently inserted into said third fluid conduit at a reference location along said third fluid conduit adjacent said valve when said stream is flowing in said reverse direction; and

stopping said flow of said stream at a point in time when said interface is detected adjusted by a delay, said delay being normalized around a predetermined nominal center point delay according to a feedback loop.

10. A method according to claim 9, further comprising actuating said valve after said flow of said stream is stopped.

11. A method according to claim 9, wherein said valve comprises a shear valve.

12. A method according to claim 9, said feedback loop being based upon a time difference TD equal to T₂−

T₁, wherein T₁ is a time at which a first particular liquid segment of a particular liquid package reaches a reference point during said flow of said stream in said forward direction and T₂ is a time at which a second particular liquid segment of said particular liquid package reaches said reference point during said flow of said stream in said reverse direction.

13. A method according to claim 12, said delay being calculated according to the following formula:

14. A method according to claim 9, wherein said detecting step is performed using an air and liquid interface detector positioned at said reference location.

15. A method according to claim 9, wherein the liquid and air segments aspirated in each of said cycles and inserted into said third fluid conduit each comprise a liquid package having a length, wherein said length is measured for a plurality of said liquid packages during said flow of said stream in either said forward or said reverse direction and wherein an average length is calculated using a plurality of said measured lengths, said feedback loop being based upon a difference between a target liquid package length and said average length.

16. A method according to claim 15, said delay being calculated according to the following formula: