Alteration of microbial populations in the gastrointestinal tract

• US 6,348,452 B1
• Filed: 01/29/1999
• Issued: 02/19/2002
• Est. Priority Date: 03/20/1996
• Status: Expired due to Term

• Current Assignee: Corn Products International Incorporated (Ingredion Incorporated)
• Original Assignee: Arnott's Biscuits Limited, Burns Philp & Company, Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education), Gist-Brocades Australia PTY Limited (DSM-Firmenich AG), AB Mauri Technology & Development Pty Ltd. (Garfield Weston Foundation), Goodman Fielder Ingredients Limited, University Of New South Wales
• Inventors: Wang, Xin, Conway, Patricia Lynne, McNaught, Kenneth J., Evans, Anthony John, Brown, Ian L., Henriksson, Karl Anders Olof
• Primary Examiner(s): Weddington, Kevin E.
• Application Number:
  US09/155,116
• Time in Patent Office: 1,117 Days
• Field of Search: 514/60, 424/93.4
• US Class Current: 514/60
• CPC Class Codes: A23L 29/212 Starch; Modified starch; Starch derivatives, e.g. esters or ethers containing starch hydrolysates, e.g. dextrin, A23L29/30
• CPC Class Codes: A23L 29/219 Chemically modified starch; Reaction or complexation products of starch with other chemicals
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 9/1652 Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin homeopathic globules A61K9/1623
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/14 Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Method of enhancing a resident population of microorganism in a selected site of the gastrointestinal tract of an animal, the method comprising providing to the animal a selected modified or unmodified resistant starch or mixtures thereof in combination with one or more probiotic microorganisms such that upon ingestion the starch passes through the gastrointestinal tract substantially unutilized until it reaches the selected site where it is utilized by the resident and/or the probiotic microorganisms thereof causing an increase in number and/or activity of the microorganisms.

Claims

1. A method of altering the number, activity, or number and activity of a resident population of microorganism in a selected site of the gastrointestinal tract of an animal, the method comprising providing to the animal a selected modified or unmodified resistant starch or mixtures thereof in the form of high amylose starch selected from the group consisting of maize starch having an amylose content of 50% w/w or more, rice starch having an amylose content of 27% w/w or more, and wheat starch having an amylose content of 27% w/w or more, in combination with one or more probiotic microorganism such that upon ingestion the resistant starch passes through the gastrointestinal tract substantially unutilized until reaching the selected site of the gastrointestinal site where the resistant starch is utilized by the resident population of microorganism, the probiotic microorganisms or both the resident population and probiotic microorganisms thereby causing an alteration in number, activity, or number and activity of the resident population of microorganism.

2. The method according to claim 1 wherein the maize starch has an amylose content of 80% w/w or more.

3. The method according to claim 1 wherein the maize starch comprises particular granular size ranges of starches having enhanced resistant starch content.

4. The method according to claim 1 wherein the high amylose starch is modified chemically, enzymatically, physically, or mixture thereof.

5. The method according to claim 4 wherein the chemical modification is by cross-bonding, etherification, esterification, or acidification.

6. The method according to claim 4 wherein the physical modification is by crystallization.

7. The method according to claim 1 wherein the modified resistant starch is selected from the group consisting of hydroxypropylated starch, acetylated starch, octenyl succinated starch, carboxymethylated starch, and succinated starch.

8. The method according to claim 1 wherein the number, activity, or number and activity of the resistant population of microorganism is increased.

9. The method according to claim 1 wherein the number, activity, or number and activity of the resident population of microorganism is decreased.

10. The method according to claim 9 wherein the resident population of microorganism is a microbial pathogen.

11. The method according to claim 10 wherein the resistant starch acts as a carrier which functions to transport the one or more probiotic microorganisms to the selected site of the gastrointestinal tract, and which carrier acts as a growth or maintenance medium for non-pathogenic microorganism in the selected site of the gastrointestinal tract to an extent sufficient to suppress growth, activity, or growth and activity of the microbial pathogen.

12. The method according to claim 1 wherein the number of the probiotic microorganisms is increased.

13. The method according to claim 1 wherein the activity of the probiotic microorganism is increased.

14. The method according to claim 1 wherein the selected site in the gastrointestinal tract is the small intestine, stomach or large bowel.

15. The method according to claim 1 wherein the one or more probiotic microorganism, the resident population of microorganism, or the probiotic and resident population of microorganisms are bacteria which produce short chain fatty acids (SCFA).

16. The method according to claim 15 wherein SCFA is butyrate and the probiotic bacteria are Clostridium butyricum, Eubacterium, or mixture thereof.

17. The method according to claim 15 wherein the alteration in number, activity, or number and activity of the resident population of microorganism results in reducing the incidence of colorectal cancer or colonic atrophy in the animal.