×

Automated DNA array segmentation and analysis

  • US 6,349,144 B1
  • Filed: 02/07/1998
  • Issued: 02/19/2002
  • Est. Priority Date: 02/07/1998
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for segmentation of a frame of image information including a plurality of spaced DNA spot images corresponding to a plurality of DNA spots, said image information including image intensity level information corresponding to said DNA spots, the method comprising the steps of:

  • (a) storing the frame of image information in a memory device;

    (b) selecting a set of image information within said frame including a selected set of the DNA spot images;

    (c) generating a grid in said memory device, the grid including a plurality of spaced grid points corresponding to said selected DNA spot images, each grid point including position information indicating the position of the grid point within said frame; and

    (d) modifying a current position of at least one grid point corresponding to a spot image in said selected image, including the steps of;

    (i) selecting a first bounding area in the frame around the current position of the grid point;

    (ii) generating a first position update including position information for updating a current position of said grid point to a first new position within the first bounding area, the location of said first new position relative to said current position being a function of intensity level of at least a portion of the image within the first bounding area;

    (iii) generating a second position update including position information for updating said current position to a second new position in the frame, said second new position being in a geometric arrangement with the position of one or more grid points around said grid point; and

    (iv) updating said current position with the position information of the first and the second position updates, thereby shifting said grid point toward the corresponding spot image.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×