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Electrochemical and photochemical electrodes and their use

  • US 6,350,368 B1
  • Filed: 06/30/1999
  • Issued: 02/26/2002
  • Est. Priority Date: 05/27/1996
  • Status: Expired due to Fees
First Claim
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1. A process for preparing an electrode having FAD-dependent redox enzymes immobilized thereon, the process comprising:

  • (a) preparing apo-enzymes by treating an FAD-dependent enzyme so as to remove the FAD-cofactor therefrom;

    (b) preparing a functionalized FAD by covalent binding to a binding moiety capable of chemical association with, attachment to or a chemical sorption to the surface of the electrode;

    (c) reacting the functionalized FAD with an electrode under conditions such that the modified FAD becomes immobilized onto the electrode through chemical association, attachment or sorption of the binding moiety onto the surface of the electrode;

    (d) reacting the electrode obtained in (c) with the apo-enzyme under conditions in which the apo-enzyme combines with the modified FAD to yield functional immobilized enzymes; and

    (e) reacting the electrode obtained in (d) with an enzyme substrate in the presence of a diffusional electron mediator.

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