Redox reversible bipyridyl-osmium complex conjugates
First Claim
Patent Images
1. An electrochemically detectable compound of the formula whereinR and R1 are the same or different and are 2,2′
- -bipyridyl, 4,4′
-disubstituted-2,2′
-bipyridyl, 5-5′
-disubstituted,-2,2′
-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, or 5,6-disubstituted-1,10-phenanthrolinyl, wherein each substituent is a methyl, ethyl, or phenyl group, R5 is 4-substituted-2,2′
-bipyridyl or 4,4′
-disubstituted-2,2′
-bipyridyl wherein the 4-substituent is the group B—
(L)k—
Q(CH2)i— and
the 4′
-substituent is a methyl, ethyl or phenyl group;
R, R1 and R5 are coordinated to Os through their nitrogen atoms;
Q is O, S, or NR4 wherein R4 is hydrogen, methyl or ethyl;
—
L—
is a divalent linker;
k is 1 or 0;
i is 1, 2, 3, 4, 5 or 6;
B is hydrogen or a group comprising a ligand that binds to a specific binding partner;
d is +2 or +3;
X and Y are anions selected from monovalent anions chloride, bromide, iodide, fluoride, tetrafluoroborate, perchlorate, and nitrate and divalent anions sulfate, carbonate or sulfite wherein x and y are independently 0, 1, 2, or 3 so that the net charge of XxYy is −
2 or −
3.
4 Assignments
0 Petitions
Accused Products
Abstract
Novel bipyridyl-osmium complex conjugates, their preparation, and their use in electrochemical assays are described. The redox reversible-osmium complexes can be prepared to exhibit unique reversible redox potentials and can thus be used in combination with other electroactive redox reversible species having redox potentials differing by at least 50 millivolts in electrochemical assays designed for use of multiple electroactive species in the same cell and in the same sample without interference between the two or more redox coupled conjugate systems.
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Citations
8 Claims
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1. An electrochemically detectable compound of the formula
wherein R and R1 are the same or different and are 2,2′ - -bipyridyl, 4,4′
-disubstituted-2,2′
-bipyridyl, 5-5′
-disubstituted,-2,2′
-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, or 5,6-disubstituted-1,10-phenanthrolinyl, wherein each substituent is a methyl, ethyl, or phenyl group,R5 is 4-substituted-2,2′
-bipyridyl or 4,4′
-disubstituted-2,2′
-bipyridyl wherein the 4-substituent is the group B—
(L)k—
Q(CH2)i— and
the 4′
-substituent is a methyl, ethyl or phenyl group;
R, R1 and R5 are coordinated to Os through their nitrogen atoms;
Q is O, S, or NR4 wherein R4 is hydrogen, methyl or ethyl;
—
L—
is a divalent linker;
k is 1 or 0;
i is 1, 2, 3, 4, 5 or 6;
B is hydrogen or a group comprising a ligand that binds to a specific binding partner;
d is +2 or +3;
X and Y are anions selected from monovalent anions chloride, bromide, iodide, fluoride, tetrafluoroborate, perchlorate, and nitrate and divalent anions sulfate, carbonate or sulfite wherein x and y are independently 0, 1, 2, or 3 so that the net charge of XxYy is −
2 or −
3.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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8. The detectable compound of claim 6 wherein B comprises a of the formula
- -bipyridyl, 4,4′
Specification