Method of measuring substance in sample using a redox reaction
First Claim
1. A method of measuring an analyte in a sample using a redox reaction, comprising:
- eliminating the influence of any reducing substance contained in the sample prior to conducting the redox reaction, the influence of any reducing substance being eliminated by adding a tetrazolium compound prior to the redox reaction;
forming a reducing substance or an oxidizing substance derived from the analyte;
measuring the quantity of the formed substance derived from the analyte by the redox reaction; and
determining the quantity of the analyte from the quantity of the formed substance derived from the analyte;
wherein the tetrazolium compound used to eliminate the influence of any reducing substance comprises at least one of 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium salt, 3,3′
-(1,1′
-biphenyl-4,4′
-diyl)-bis(2,5-diphenyl)-2H-tetrazolium salt, 3,3′
-[3,3′
-dimethoxy-(1,1′
-biphenyl)-4,4′
-diyl]-bis[2-(4-nitropenyl)-5-phenyl-2H-tetrazolium salt, 2,3-diphenyl-5-(4-chlorophenyl)tetrazolium salt, 2,5-diphenyl-3-(p-diphenyl)tetrazolium salt, 2,3-diphenyl-5-(p-diphenyl)tetrazolium salt, 2,5-diphenyl-3-(4-styrylphenyl)tetrazolium salt, 2,5-diphenyl-3-(m-tolyl)tetrazolium salt, and 2,5-diphenyl-3-(p-tolyl) tetrazolium salt.
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Accused Products
Abstract
A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.
38 Citations
20 Claims
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1. A method of measuring an analyte in a sample using a redox reaction, comprising:
-
eliminating the influence of any reducing substance contained in the sample prior to conducting the redox reaction, the influence of any reducing substance being eliminated by adding a tetrazolium compound prior to the redox reaction;
forming a reducing substance or an oxidizing substance derived from the analyte;
measuring the quantity of the formed substance derived from the analyte by the redox reaction; and
determining the quantity of the analyte from the quantity of the formed substance derived from the analyte;
wherein the tetrazolium compound used to eliminate the influence of any reducing substance comprises at least one of 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium salt, 3,3′
-(1,1′
-biphenyl-4,4′
-diyl)-bis(2,5-diphenyl)-2H-tetrazolium salt, 3,3′
-[3,3′
-dimethoxy-(1,1′
-biphenyl)-4,4′
-diyl]-bis[2-(4-nitropenyl)-5-phenyl-2H-tetrazolium salt, 2,3-diphenyl-5-(4-chlorophenyl)tetrazolium salt, 2,5-diphenyl-3-(p-diphenyl)tetrazolium salt, 2,3-diphenyl-5-(p-diphenyl)tetrazolium salt, 2,5-diphenyl-3-(4-styrylphenyl)tetrazolium salt, 2,5-diphenyl-3-(m-tolyl)tetrazolium salt, and 2,5-diphenyl-3-(p-tolyl) tetrazolium salt.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of measuring an analyte in a sample using a redox reaction, comprising:
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eliminating the influence of any reducing substance contained in the sample prior to conducting the redox reaction, the influence of any reducing substance being eliminated by adding a tetrazolium compound prior to the redox reaction;
forming an oxidizing substance comprising hydrogen peroxide that is derived from the analyte;
measuring the quantity of the hydrogen peroxide derived from the analyte by the redox reaction with a peroxidase and a substrate that develops color when oxidized, the substrate comprising N-(carboxymethylaminocarbonyl)-4,4′
-bis(dimethylamino)diphenylamine sodium; and
determining the quantity of the analyte from the quantity of the formed substance derived from the analyte. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
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Specification