Remotely programmable matrices with memories

• US 6,352,854 B1
• Filed: 06/07/1995
• Issued: 03/05/2002
• Est. Priority Date: 04/25/1995
• Status: Expired due to Term

• Current Assignee: Irori Technologies Incorporated
• Original Assignee: Discovery Partners International, Inc. (Infinity Pharmaceuticals, Inc.)
• Inventors: Nova, Michael P., Senyei, Andrew E.
• Primary Examiner(s): Zitomer, Stephanie W.
• Application Number:
  US08/480,147
• Time in Patent Office: 2,463 Days
• Field of Search: 435/6, 435/7.1, 435/163, 435/287.1, 435/287.3, 436/161, 536/24-- 3, 382/178-179, 422/68.1, 530/300, 530/350, 340/572
• US Class Current: 506/16
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00295 the reactor vessels having pervious side walls
• CPC Class Codes: B01J 2219/00299 Generally cylindrical reactor vessels
• CPC Class Codes: B01J 2219/00308 interchangeably mounted in racks or blocks
• CPC Class Codes: B01J 2219/00344 Caps
• CPC Class Codes: B01J 2219/00461 Beads and reaction vessel together
• CPC Class Codes: B01J 2219/00463 Directed sorting
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00502 Particles of irregular geometry
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00542 Alphanumeric characters
• CPC Class Codes: B01J 2219/00549 2-dimensional
• CPC Class Codes: B01J 2219/00551 3-dimensional
• CPC Class Codes: B01J 2219/0056 Raised or sunken areas
• CPC Class Codes: B01J 2219/00567 Transponder chips
• CPC Class Codes: B01J 2219/00569 EEPROM memory devices
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00592 Split-and-pool, mix-and-divide processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00689 : using computers
• CPC Class Codes: B01J 2219/00695 : Synthesis control routines, e.g. using computer programs
• CPC Class Codes: B01J 2219/0072 : Organic compounds
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B82Y 10/00 : Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: C07K 1/00 : General methods for the preparation of peptides , i.e. processes for the organic chemical preparation of peptides or proteins of any length
• CPC Class Codes: C07K 1/04 : on carriers C07K1/003, C07K1/006 take precedence
• CPC Class Codes: C07K 1/047 : Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 15/149 : specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2035/00782 : reprogrammmable code
• CPC Class Codes: G01N 35/00732 : Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: G01N 35/00871 : Communications between instruments or with remote terminals
• CPC Class Codes: G11C 13/0014 : comprising cells based on organic memory material
• CPC Class Codes: G11C 13/0019 : comprising bio-molecules
• CPC Class Codes: G11C 13/025 : using fullerenes, e.g. C60, or nanotubes, e.g. carbon or silicon nanotubes

Abstract

Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The data storage units are preferably non-volatile antifuse memories. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, chemical modification and other uses.

Claims

1. A combination of a matrix with memory, comprising:

(a) a memory device, comprising a data storage unit, wherein the memory device is remotely programmable and/or remotely readable using transmitted electromagnetic radiation; and

(b) a matrix material comprising particles of a size such that at least one dimension is no more than 100 mm, wherein the matrix material is derivatized for linking biological particles or molecules, the biological particles comprising a virus, viral vector, viral capsid with or without packaged nucleic acid, phage, phage vector or phage capsid with or without encapsulated nucleic acid, a eukaryotic or prokaryotic cell or fragments of the virus, viral capsid, phage, phage capsid or cell, and the molecules comprising macromolecules or monomeric constituents of macromolecules or organic compounds;

wherein the memory device is in contact with the matrix material or in a solution that is in contact with the matrix material.

2. The combination of claim 1, wherein the memory device comprises a non-volatile memory means for storing a plurality of data points and means for receiving a transmitted write signal so that the write signal causes a stored data point corresponding to the data signal to be stored within the non-volatile memory means.

3. The combination of claim 1, wherein the memory device further comprises a plurality of memory address locations for storing a plurality of data points therein, wherein each of the memory address locations is uniquely addressable.

4. The combination of claim 1, wherein the memory device comprises an optical storage means for storing a plurality of data points as a plurality of holes or pits.

5. The combination of claim 1, wherein the memory device further comprises an antenna for receiving the transmitted electromagnetic radiation.

6. The combination of claim 1, wherein the memory device further comprises a porous shell enclosing the memory device and particles, the porous shell comprising a material that is inert with respect to the memory device and transmissive of the electromagnetic radiation.

7. The combination of claim 1, further comprising a molecule or a biological particle linked to the matrix material.

8. The combination of claim 1, wherein the memory device is encased in the matrix material.

9. The combination of claim 1, wherein at least one surface of the memory device is coated with the matrix material.

10. The combination of claim 1, wherein the matrix material is selected from among a polystyrene, a cellulose, a glass, a polyacrylamide, a polysaccharide, a rubber, silicon, a plastic, sand, pumice, agarose, halogenated hydrocarbon polymers, and any polymer used as a matrix in solid phase syntheses.

11. The combination of claim 1, wherein the memory device comprises a non-volatile memory means.

12. The combination of claim 11, wherein the non-volatile memory means comprises an antifuse.

13. In a library comprised of molecules or biological particles, wherein the constituent molecules are biological particles are combined with a solid support matrix, the improvement comprising, combining the solid support matrix with a memory device containing a data storage unit, wherein the memory device is remotely programmable and/or remotely readable using transmitted electromagnetic radiation.

14. The library of claim 13 that is a combinatorial library.

15. The library of claim 13, wherein the components of the library are molecules that are peptides, peptoids, oligonucleotides or organic molecules.

16. The library of claim 13 that is a phage display library.

17. The library of claim 13, wherein the memory device is in contact with the solid support material.

18. The library of claim 13, wherein the memory device is on the surface of the solid support material.

19. The library of claim 13, wherein the solid support material is in the form of a tube that encases the recording device.

20. The library of claim 13, wherein the solid support material is derivatized for linking biological particles or molecules.

21. The library of claim 13, wherein the solid support material is selected from among a polystyrene, a cellulose, a glass, a polyacrylamide, a polysaccharide, a rubber, silicon, a plastic, sand, pumice, agarose, halogenated hydrocarbon polymers, and any polymer used as a matrix in solid phase syntheses.

22. The library of claim 13, wherein the memory device comprises an optical storage means for storing a plurality of data points as a plurality of holes or pits.

23. The library of claim 13, wherein the memory device further comprises an antenna for receiving the transmitted electromagnetic radiation.

24. The library of claim 13, wherein the solid support matrix comprises a plurality of particles and the memory device further comprises a porous shell enclosing the memory device and particles, the porous shell comprising a material that is inert with respect to the memory device and transmissive of the electromagnetic radiation.

25. The library of claim 13, wherein the memory device comprises a non-volatile memory means for storing a plurality of data points and means for receiving a transmitted write signal so that the write signal causes a stored data point corresponding to the data signal to be stored within the non-volatile memory means.