Light source power modulation for use with chemical and biochemical analysis

• US 6,353,475 B1
• Filed: 08/19/1999
• Issued: 03/05/2002
• Est. Priority Date: 07/12/1999
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc., Caliper Technologies Corp (Revvity, Inc.)
• Original Assignee: Agilent Technologies, Inc., Caliper Technologies Corp (Revvity, Inc.)
• Inventors: Jensen, Morten J., Brombacher, Volker, Kaltenbach, Patrick
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): Punnoose, Roy M.
• Application Number:
  US09/377,891
• Time in Patent Office: 929 Days
• Field of Search: 356/244, 356/344, 250/458.1
• US Class Current: 356/244
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

Systems and methods for analyzing a sample are disclosed. The system may include a light source operable to transmit light onto the sample, a detector operable to detect intensity of the light emitted from the sample, and a power modulator. The power modulator modulates the light source power such that light is emitted from the light source in more than one mode to reduce changes in the emitted light due to temperature changes in the light source.

Claims

1. A method of analyzing a sample comprising:

positioning the sample within an optical path of a light source;

modulating power to the light source such that light is emitted from the light source in more than one mode to reduce changes in the emitted light due to temperature changes in the light source;

detecting intensity of light emitted from the sample upon exposure to the light source with a detector; and

removing effects of modulating power to the light source from an output signal of the detector.

2. The method of claim 1 wherein positioning the sample comprises positioning a microfluidic device containing the sample within the optical path.

3. The method of claim 2 wherein positioning the sample further comprises electrokinetically transporting the sample within the microfluidic device.

4. The method of claim 1 wherein modulating power to the light source comprises turning the light source on for more than 50% of the time.

5. The method of claim 4 wherein modulating the power to the light source further comprises turning the light source on for more than 80% of the time.

6. The method of claim 5 wherein modulating the power to the light source further comprises turning the light source on for approximately 89% of the time.

7. The method of claim 1 further comprising regulating the power provided to the light source to provide a generally constant power output from the light source when the light source is on.

8. The method of claim 1 wherein removing effects of modulating power to the light source comprises filtering the output signal of the detector to substantially remove power modulation frequency from the output signal.

9. The method of claim 1 wherein the light source is a laser.

10. The method of claim 9 wherein the light source is a laser diode.

11. The method of claim 1 further comprising sending data representative of the intensity of the light to a microprocessor to decode the data.

12. The method of claim 11 wherein sending data comprises electronically filtering the data to eliminate fluctuations due to modulation of the light source power.

13. The method of claim 1 wherein modulating the power comprises modulating the power between approximately zero milliwatts and ten milliwatts.

14. The method of claim 9 wherein removing effects of modulating power to the light source comprises synchronizing a laser modulation signal with a detector sampling time to substantially remove traces of laser modulation in the output signal.

15. A system for use in analyzing a sample, comprising:

a light source operable to transmit light onto the sample;

a detector operable to detect intensity of light emitted from the sample; and

a power modulator operable to modulate light source power such that light is emitted from the light source in more than one mode to reduce changes in the emitted light due to temperature changes in the light source, wherein effects of modulated light source power are removed from an output signal of the detector.

16. The system of claim 15 wherein the power modulator is operable to modulate the power at a duty cycle of approximately 89%.

17. The system of claim 16 wherein the power modulator is operable to turn the power on for approximately eight microseconds and off for approximately one microsecond.

18. The system of claim 15 wherein the power modulator is operable to cycle the power between approximately zero milliwatts and approximately ten milliwatts.

19. The system of claim 15 wherein the detector is a fluorescent detector.

20. The system of claim 15 further comprising a microprocessor for receiving data from the detector and decoding the data.

21. The system of claim 15 further comprising a power regulator operable to regulate the light source to provide a generally constant output power when the light source is on.

22. The system of claim 21 further comprising a reference detector operable to receive a sample of light from the light source and send a feedback signal to the power regulator to maintain a generally constant output power at the light source.

23. The system of claim 15 wherein the light source is a laser.

24. The system of claim 23 wherein the light source is a laser diode.

25. The system of claim 15 further comprising a beamsplittcr for filtering a portion of light emitted from the light source and reflecting a portion of light emitted from the sample.

26. The system of claim 15 further comprising a filter operable to filter the output signal from the detector to substantially remove power modulation frequency from the output signal.

27. The system of claim 23 further comprising a synchronization device operable to synchronize a laser modulation signal with a detector sampling time.

28. A system for use in analyzing a sample, comprising:

a microfluidic device;

a light source operable to transmit light onto the sample positioned within the microfluidic device;

a detector operable to detect intensity of the light emitted from the sample;

a power modulator operable to modulate light source power such that light is emitted from the light source in more than one mode to reduce changes in the emitted light due to temperature changes in the light source; and

a filter operable to filter the output signal from the detector to substantially remove effects of modulated light source power.

29. The system of claim 28 wherein the microfluidic device comprises a detection region and a plurality of channels in fluid communication with the detection region.

30. The system of claim 29 wherein the microfluidic device comprises a material transport system for transporting the sample through at least one of the plurality of channels to the detection region.

31. The system of claim 30 wherein the material transport system is an electrokinetic transport system.

32. The system of claim 30 wherein the material transport system is a pressure based system.

33. The system of claim 28 wherein the power modulator is operable to modulate the power at a duty cycle of approximately 89%.

34. The system of claim 33 wherein the power modulator is operable to turn the power on for approximately eight microseconds and off for approximately one microsecond.

35. The system of claim 28 wherein the detector is a fluorescent detector.

36. The system of claim 28 wherein the light source is a laser.

37. The system of claim 28 further comprising a microprocessor for receiving data from the detector and decoding the data.

38. The system of claim 28 further comprising a power regulator operable to regulate the light source to provide a generally constant power output when the light source is on.