Methods and products for analyzing polymers
First Claim
Patent Images
1. A method for analyzing a polymer of linked units comprisingmoving a plurality of individual units of a polymer of linked units through a channel and exposing the plurality of individual units to an agent selected from the group consisting of electromagnetic radiation, a quenching source and a fluorescence excitation source as the units move past the agent, individual units interacting with the agent to produce a detectable signal within the channel or at the edge of the channel, and detecting sequentially the signals resulting from said interaction to analyze the polymer.
6 Assignments
0 Petitions
Accused Products
Abstract
Methods and products for analyzing polymers are provided. The methods include methods for determining various other structural properties of the polymers.
-
Citations
123 Claims
-
1. A method for analyzing a polymer of linked units comprising
moving a plurality of individual units of a polymer of linked units through a channel and exposing the plurality of individual units to an agent selected from the group consisting of electromagnetic radiation, a quenching source and a fluorescence excitation source as the units move past the agent, individual units interacting with the agent to produce a detectable signal within the channel or at the edge of the channel, and detecting sequentially the signals resulting from said interaction to analyze the polymer.
-
29. A method for analyzing a polymer of linked units comprising:
-
moving a plurality of individual units of a polymer of linked units through a channel and past a station; and
,detecting sequentially signals arising from a detectable physical change in the polymer or the station as individual units pass the station within the channel or at the edge of the channel to analyze the polymer. - View Dependent Claims (30, 31, 120, 123)
-
-
32. A method for analyzing a polymer of linked units, comprising:
exposing a plurality of individual units of a polymer to a station to produce a non-ion conductance signal resulting from the exposure of the units of the polymer to the station, and wherein the station is attached to a wall material having a surface defining a channel, and wherein the polymer is not fixed to the surface. - View Dependent Claims (84, 85, 86, 87, 121)
-
33. An apparatus for analyzing a polymer of linked units by detecting a signal comprising
an interaction station positioned within a channel constructed and arranged to receive a polymer of linked units sequentially passing through said station, selected units of said polymer being labeled with a light sensitive label; -
a source of electromagnetic radiation constructed and arranged to irradiate, at said interaction station, said labeled units;
a sensor constructed and arranged to detect sequentially electromagnetic radiation providing characteristic signals resulting from interaction with said label or said unit; and
a processor constructed and arranged to analyze said polymer based on said sequentially detect radiation. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 80, 119)
-
-
49. A method for identifying an individual unit of a polymer comprising
transiently exposing the individual unit of the polymer to an agent selected from the group consisting of electromagnetic radiation, a quenching source and a fluorescence excitation source, the identity of the individual unit being unknown, to generate an interaction with a detectable electromagnetic radiation signal characteristic of said individual unit, detecting said electromagnetic radiation signal, and distinguishing said signal from signals generated from adjacent signal generating units of the polymer as an indication of the identity of the individual unit.
-
50. A method for determining the order of two individual units of a polymer of linked units comprising:
-
involving the polymer of linked units linearly with respect to a station, exposing one of the individual units to the station to produce a signal arising from a detectable physical change in the unit or the station, exposing the other of the individual units to the station to produce a second detectable signal arising from a detectable physical change in the unit or the station, different from the first signal, wherein at least one of the two individual units is an internal unit and wherein at least one of the two individual units has an extrinsic label; and
determining the order of the signals as an indication of the order of the two individual units. - View Dependent Claims (92, 93, 94, 95, 96, 97, 98, 118)
-
-
51. A method for analyzing a polymer of linked units comprising:
-
(1) providing a polymer of linked units, labeled with a unit specific marker (2) detecting sequentially signals from unit specific markers of less than all of the linked units that the unit specific markers are capable of identifying, and (3) storing a signature of said signals detected to analyze the polymer. - View Dependent Claims (99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114)
-
-
52. A method for analyzing a polymer of linked units comprising:
-
labeling selected units of said polymer with light sensitive labels and passing said polymer through a channel;
irradiating said labeled units of said straightened polymer with electromagnetic radiation of a selected wavelength, detecting sequentially electromagnetic radiation providing characteristic signals resulting from interaction with said labels or said units, and analyzing said polymer based on said detected radiation. - View Dependent Claims (53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 115, 116, 122)
contacting a dividing cell with a nucleotide analog, isolating from the cell nucleic acids that have incorporated the nucleotide analog, and modifying the nucleic acid with incorporated nucleotide analog by labeling the incorporated nucleotide analog. -
77. The method of claim 76 wherein said nucleotide analog is a brominated analog.
-
78. The method of claim 77 wherein said dividing cell is contacted with a nucleotide analog by growth arresting the cell in the cell division cycle, performing the contacting step, and allowing the cell to reenter the cell division cycle.
-
79. The method of claim 75 wherein the nucleic acids are isolated after said cells have reentered and completed said cell division cycle and before a second cell division cycle is completed.
-
115. The method of claim 52, wherein the channel is a nanochannel.
-
116. The method of claim 52, wherein the channel is a microchannel.
-
122. The method of claim 52, wherein the light sensitive labels are on a unit specific marker and wherein the polymer is labeled with the unit specific marker.
-
-
83. A method for analyzing a polymer of linked units comprising:
-
moving a plurality of individual units of a polymer of linked units with respect to a station; and
,detecting sequentially signals arising from a detectable physical change in the polymer or the station as individual units pass the station to analyze a polymer, and wherein the signal is not an electromagnetic radiation signal.
-
Specification