Cytokine designated 4-1BB ligand antibodies and human receptor that binds thereto

• US 6,355,779 B1
• Filed: 09/10/1998
• Issued: 03/12/2002
• Est. Priority Date: 05/07/1993
• Status: Expired due to Fees

• Current Assignee: Immunex Corporation (Amgen Inc.)
• Original Assignee: Immunex Corporation (Amgen Inc.)
• Inventors: Alderson, Mark R., Smith, Craig A., Goodwin, Raymond G.
• Primary Examiner(s): Eyler, Yvonne
• Assistant Examiner(s): BRANNOCK, MICHAEL T
• Application Number:
  US09/150,864
• Time in Patent Office: 1,279 Days
• Field of Search: 530/388.23, 530/350, 530/351, 530/388.22, 436/501, 435/7.1, 435/7.2, 435/69.1, 435/252.3, 536/23.5, 536/24.31
• US Class Current: 530/388.23
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/52 Cytokines; Lymphokines; Interferons
• CPC Class Codes: C07K 14/715 for cytokines; for lymphokines; for interferons
• CPC Class Codes: C07K 2319/00 Fusion polypeptide

Abstract

Novel 4-1BB ligand (4-1BB-L) polypeptides and a human cell surface receptor designated 4-1BB that binds 4-1BB-L are provided. Isolated 4-1BB-L-encoding and human 4-1BB-encoding DNA sequences, recombinant expression vectors comprising the isolated DNA sequences, and host cells transformed with the recombinant expression vectors are disclosed, along with methods for producing the novel polypeptides by cultivating such transformed host cells. Soluble forms of the 4-1BB-L or 4-1BB polypeptides are derived from the extracellular domains thereof.

Claims

1. An antibody that specifically binds a polypeptide selected from the group consisting of:

(a) a polypeptide encoded by a DNA consisting of nucleotides 53-979 of SEQ ID NO;

1 or nucleotides 4-765 of the sequence of SEQ ID NO;

3;

(b) a polypeptide encoded by a DNA which, due to the degeneracy of the genetic code, encodes a polypeptide encoded by a DNA of (a).

2. An antibody of claim 1, wherein said antibody is a monoclonal antibody.

3. An antibody that specifically binds a polypeptide consisting of amino acids 1-254 of SEQ ID NO:

4, amino acids 49-254 of SEQ ID NO;

4, amino acids 1-309 of SEQ ID NO;

2, or amino acids x-309 of SEQ ID NO;

2, wherein x is selected from the group consisting of 104, 105 and 106.

4. An antibody of claim 3, wherein said antibody is a monoclonal antibody.