Device and method for isolating cell material out of a tissue medium and/or a liquid
First Claim
1. An apparatus for isolating cell material from at least one of a tissue system or a liquid, comprising:
- a vessel open at the top into which cell material in the tissue system or in the liquid can be introduced; and
an isolating device comprising;
an actuating shaft; and
a flat isolating disk having grinding elements on an underside of the disk facing the bottom of said vessel;
having a peripheral edge flush with inner walls of the vessel in a fluid-tight manner; and
having at least one passage opening covered by a filter membrane, and which can be inserted into said vessel from the top;
wherein said grinding elements introduce shearing forces into the cell material as said isolating disk rotates.
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Accused Products
Abstract
An apparatus for isolating cell material from a tissue system and/or a liquid includes (1) a vessel open at the top, into which the cell material to be isolated can be introduced in the tissue system and/or the liquid; and (2) a separating device having a stamp-shaped configuration and including a flat separating disk having a peripheral edge fitting with the inner walls of the vessel in a fluid-tight manner and presenting at least one passage opening which is covered by a filter membrane, and which device can be inserted from the top into the vessel. The isolating disk pressurises the cell material inclusive of the tissue system and/or the liquid and acts thereupon with shearing forces by rotation. The cells and/or cell systems pass through the pores of the filter membrane whereas the residual tissue material is retained.
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Citations
16 Claims
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1. An apparatus for isolating cell material from at least one of a tissue system or a liquid, comprising:
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a vessel open at the top into which cell material in the tissue system or in the liquid can be introduced; and
an isolating device comprising;
an actuating shaft; and
a flat isolating disk having grinding elements on an underside of the disk facing the bottom of said vessel;
having a peripheral edge flush with inner walls of the vessel in a fluid-tight manner; and
having at least one passage opening covered by a filter membrane, and which can be inserted into said vessel from the top;
wherein said grinding elements introduce shearing forces into the cell material as said isolating disk rotates. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. An apparatus for isolating cell material from at least one of a tissue system or a liquid, consisting of:
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a vessel open at the top into which cell material in the tissue system or in the liquid can be introduced; and
an isolating device comprising;
an actuating shaft and a flat isolating disk having grinding elements and having a peripheral edge flush with inner walls of the vessel in a fluid-tight manner and having at least one passage opening covered by a filter membrane, and which can be inserted into said vessel from the top;
wherein said grinding elements introduce shearing forces into the cell material as said isolating disk rotates.
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Specification