Device and method for isolating cell material out of a tissue medium and/or a liquid

• US 6,358,474 B1
• Filed: 04/26/2000
• Issued: 03/19/2002
• Est. Priority Date: 07/08/1997
• Status: Expired due to Fees

• Current Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Original Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Inventors: Kiesewetter, Stefan, Kuhn, Claus, Tolle, Gabriele, Dobler, Hannes, Bernhagen, Jürgen, Lindner, Hans, Tovar, Günter
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): SINES, BRIAN J
• Application Number:
  US09/462,409
• Time in Patent Office: 692 Days
• Field of Search: 435/286, 435/311, 435/270, 435/1.3, 435/267, 435/297.1, 210/87, 210/236, 210/669, 422/99
• US Class Current: 422/535
• CPC Class Codes: C12M 45/02 by mechanical forces; Stirring; Trituration; Comminuting crushing, pulverizing, disintegrating in general B02C
• CPC Class Codes: G01N 1/286 involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising microtomes G01N1/06; pulverising in general B02C; mixing in general B01F
• CPC Class Codes: G01N 1/34 Purifying; Cleaning processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids

Abstract

An apparatus for isolating cell material from a tissue system and/or a liquid includes (1) a vessel open at the top, into which the cell material to be isolated can be introduced in the tissue system and/or the liquid; and (2) a separating device having a stamp-shaped configuration and including a flat separating disk having a peripheral edge fitting with the inner walls of the vessel in a fluid-tight manner and presenting at least one passage opening which is covered by a filter membrane, and which device can be inserted from the top into the vessel. The isolating disk pressurises the cell material inclusive of the tissue system and/or the liquid and acts thereupon with shearing forces by rotation. The cells and/or cell systems pass through the pores of the filter membrane whereas the residual tissue material is retained.

Claims

1. An apparatus for isolating cell material from at least one of a tissue system or a liquid, comprising:

a vessel open at the top into which cell material in the tissue system or in the liquid can be introduced; and

an isolating device comprising;

an actuating shaft; and

a flat isolating disk having grinding elements on an underside of the disk facing the bottom of said vessel;

having a peripheral edge flush with inner walls of the vessel in a fluid-tight manner; and

having at least one passage opening covered by a filter membrane, and which can be inserted into said vessel from the top;

wherein said grinding elements introduce shearing forces into the cell material as said isolating disk rotates.

2. An apparatus according to claim 1, further comprising a closure cap that closes said vessel in a fluid-tight manner.

3. An apparatus according to claim 1, wherein said isolating disk pressurises said cell material.

4. An apparatus according to claim 1, wherein said isolating device comprises an actuating shaft centrally disposed on said isolating disk.

5. An apparatus according to claim 1, wherein said isolating device comprises an actuating shaft having the shape of a hollow cylinder which is flush with the peripheral edge.

6. An apparatus according to claim 5, wherein said isolating device is adapted to be driven via said actuating shaft for at least one of rotational or vertical movement.

7. An apparatus according to claim 5, wherein an upper end of said actuating shaft is adapted to be detachably fastened or fixedly mounted on said closure cap.

8. An apparatus according to claim 5, wherein said actuating shaft comprises a flange for automatic or manual actuation by a handling device.

9. An apparatus according to claim 2, wherein said closure cap is a screw-on cap.

10. An apparatus according to claim 2, wherein said closure cap comprises a septum for piercing by means of cannulae.

11. An apparatus according to claim 1, wherein said vessel is made of a synthetic material.

12. An apparatus according to claim 1, wherein said filter membrane comprises pores that ensure passage of individual cells or cell systems through the membrane and that retain remaining tissue material.

13. An apparatus according to claim 12, wherein said pores have a size of approximately 100 μ

m.

14. An apparatus according to claim 1, wherein said flat isolating disk has a plurality of openings that are covered by the filter membrane.

15. An apparatus according to claim 1, wherein said grinding elements are pointed edges.

16. An apparatus for isolating cell material from at least one of a tissue system or a liquid, consisting of:

a vessel open at the top into which cell material in the tissue system or in the liquid can be introduced; and

an isolating device comprising;

an actuating shaft and

a flat isolating disk having grinding elements and having a peripheral edge flush with inner walls of the vessel in a fluid-tight manner and having at least one passage opening covered by a filter membrane, and which can be inserted into said vessel from the top;

wherein said grinding elements introduce shearing forces into the cell material as said isolating disk rotates.