Sterilizable cultivation system with separately attachable microfiltration membrane

• US 6,358,731 B1
• Filed: 10/20/1999
• Issued: 03/19/2002
• Est. Priority Date: 10/20/1999
• Status: Expired due to Term

• Current Assignee: Wei K. Hsu
• Original Assignee: Wei K. Hsu
• Inventors: Hsu, Wei K.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/422,030
• Time in Patent Office: 881 Days
• Field of Search: 47/11, 435/297.1, 435/297.5, 435/304.1
• US Class Current: 435/297.5
• CPC Class Codes: C12M 23/14 Bags
• CPC Class Codes: C12M 23/24 Gas permeable parts
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: C12M 29/20 Degassing; Venting; Bubble traps means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02
• CPC Class Codes: C12M 37/02 Filters

Abstract

A sterilizable cultivation system is provided for growing selected organisms for use in biological applications and the cultivation edible fungi. A sealable, sterilizable container holds a growth substrate in a sterile condition. A separate, adhesive vent filter is provided, which is adhereable to a vent opening in the container. The vent filter is gas permeable and allows gas exchange between the interior of the container and the ambient environment, but prevents the passage of microbes and other organisms into or out of the container. This gas exchange prevents excess carbon dioxide from accumulating in the container and allows necessary oxygen into the container in order to maintain an appropriate growth environment for the selected organisms. The vent filter may be combined with the container (a) during manufacture of new containers, or (b) in the field by the end-user on new containers or on re-cycled containers. The separately provided vent filters allow the end-user to adjust certain parameters of the growth environment within the container in the field. This is accomplished by adjusting the venting characteristics of the cultivation system, by increasing or reducing the amount and quality of the vent filters on the container.

Claims

1. A sterilizable cultivation system for growing selected organisms for use in biological applications and the cultivation of edible fungi comprising:

a sealable container having an interior for holding a growth substrate in a sterile condition, the container being gas impermeable and having at least one vent hole to allow gas exchange between the interior of the container and an outside of the container, and the growth substrate suitable for supporting growth of the selected organisms when inoculated onto the substrate; and

an adhesive breathing vent filter having an outer layer and an inner layer, the vent filter being separately adhereable to the container to cover the vent hole in the container, the outer layer being a filter material for allowing gas exchange while not allowing microbe transmission, and the inner layer being an adhesive material of non-water soluble, pressure sensitive adhesive for closely adhering the vent filter to the container and isolating the vent hole and the interior of the container from the ambient environment outside of the container.

2. The breathing vent filter of claim 1 wherein the filter material has a periphery and a central area, the periphery for receiving the adhesive material of the inner layer, the adhesive material for adhering the vent filter to the container, and the central area for covering the vent holes and allowing gas exchange and filtration.

3. The breathing vent filter of claim 2 wherein a plurality of breathing filters are arrayed at their periphery, and provided wound into a roll and in sheets, and individual vent filters are removable from an array for adhering to the container.

4. The breathing vent filter of claim 2 wherein the filter layer comprises a microfiltration membrane, the membrane having micron and smaller sized breathing pores preventing microbes from passing into or out of the container.

5. The filter layer of claim 4 wherein the microfiltration membrane, has pores ranging in size from about 1.0 μ

m to 0.05 μ

m in diameter, which pores substantially are evenly distributed on the microfiltration membrane.

6. The adhesive breathing vent filter of claim 1 wherein the adhesive material for adhering the filter to the container is releaseable from the container.

7. The adhesive breathing vent filter of claim 1 wherein the adhesive material for adhering the filter to the container is releaseable from and re-adhereable to the container.

8. The adhesive breathing vent filter of claim 1 wherein the adhesive material is covered by a release material, which release material is removable from the adhesive layer prior to adhering the filter to the container.

9. The adhesive breathing vent filter of claim 8 wherein the adhesive material is covered by a release material, which release material is selected from the group consisting of:

a silicone release paper, a release coating and other adhesive release means known to one of ordinary skill in the art.

10. The adhesive breathing vent filter of claim 2 wherein the adhesive material for adhering the filter to the container is coated on the periphery.

11. The adhesive breathing vent filter of claim 2 wherein the outer layer comprises a laminate of a microfiltration membrane and a porous support material, to give the filter increased strength and durability.

12. The container of claim 1 wherein the vent hole has a vent hole periphery, the vent hole periphery for receiving an adhesive layer for adhering the vent filter to the container and allowing gas transmission and filtration.

13. The sterilizable cultivation system of claim 1 wherein the sealable container is a bag.

14. The sealable container of claim 1 wherein the sealable container comprises a length of tubular material and is sealable at both ends of the length of tubular material.

15. The bag of claim 14 wherein at least one of the sealable ends is un-sealable and re-sealable, allowing the bag to be re-used.

16. The sealable container of claim 13 wherein the sealable container is a bag made of a semi-transparent material.

17. The sterilizable cultivation system of claim 1 wherein the sealable container is constructed of a rigid material.

18. The sterilizable cultivation system of claim 1 wherein the selected organism is any organism desired to be cultivated without competition from other organisms.

19. The sterilizable cultivation system of claim 1 wherein the selected organism is chosen from the group consisting of fungi, nematodes and any organism capable of growing on the growth substrate.

20. The adhesive breathing vent filter of claim 1 wherein the central area of the filter layer for gas transmission and filtration is modifiable by covering it with an occlusive agent, such as adhesive tape, paint or a release paper.